5-Azacytidine [DNA methyltransferase Inhibitor]
[CAT#: MOD2005ZP70]
DNA methyltransferase inhibitor. Improves stem cell reprogramming efficiency.
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Lot Number
Description
A DNA methyltransferase inhibitor. Causes insufficient methylation. Inhibits Wnt-β-catenin signaling pathway and improves stem cell reprogramming efficiency. Exhibits antiviral, antiproliferative and anticancer effects.
Synonyms
5-AzadCyD; NSC 102816; azaCyd; 5-AZC
Chemical Name
4-Amino-1-β-D-ribofuranosyl-1,3,5-tr iazin-2(1H)-one
Modulator Type
Inhibitor
Relevant Disease
Neurological Disorders
Assay Description
Unmethylated CpG islands associated with multiple genes are partially or completely methylated in tumors and can be reactivated by 5-azacytidine [1]. 5-Azacytidine can affect the weak inducer of erythroid differentiation of Friend red leukemia cells in the same concentration range and affect the activity of DNA methyltransferase [2]. The ID50 and ID90 values of 5-azacytidine inhibiting L1210 cells were 0.019 and 0.15 μg/mL, respectively [3].
Reference
[1]. Christman JK. 5-Azacytidine and 5-aza-2'-deoxycytidine as inhibitors of DNA methylation: mechanistic studies and their implications for cancer therapy. Oncogene. 2002 Aug 12;21(35):5483-95.
[2]. Creusot F, et al. Inhibition of DNA methyltransferase and induction of Friend erythroleukemia cell differentiation by 5-azacytidineand 5-aza-2'-deoxycytidine. J Biol Chem. 1982 Feb 25;257(4):2041-8.
[3]. Li LH,et al. Cytotoxicity and mode of action of 5-azacytidine on L1210 leukemia. Cancer Res. 1970 Nov;30(11):2760-9.
[2]. Creusot F, et al. Inhibition of DNA methyltransferase and induction of Friend erythroleukemia cell differentiation by 5-azacytidineand 5-aza-2'-deoxycytidine. J Biol Chem. 1982 Feb 25;257(4):2041-8.
[3]. Li LH,et al. Cytotoxicity and mode of action of 5-azacytidine on L1210 leukemia. Cancer Res. 1970 Nov;30(11):2760-9.
Assay Description
When animals were exposed to 5-Azacitidine (100 mg/kg, i.p.) for 2 hours or longer, TdR-3H incorporation was significantly inhibited [1].
References
[1]. Li LH,et al. Cytotoxicity and mode of action of 5-azacytidine on L1210 leukemia. Cancer Res. 1970 Nov;30(11):2760-9.
Molecular Weight
244.2
Form
Powder
Chemical Formula
C8H12N4O5
Source
Synthetic
SMILES
O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N2C(N=C(N)N=C2)=O
Solubility
Soluble in water (50mM) or DMSO (100mM)
CAS NO.
320-67-2
PubChem Substance ID
9444
Purity
>98%
Storage
Room temperature
Shipping
Room Temperature
Handling Advice
For obtaining a higher solubility, please warm the tube at 37 °C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -24°C for several months.
Research Use Only
For research use only
For Research Use Only. Not For Clinical Use.
