AAV-mDLX-CRE-tdTomato

Product Overview

Description

The purity of these AAV preparations is suitable for injection into animals.

Tracer Type

Recombinase

Serotype

AAV2/1; AAV2/2; AAV2/4; AAV2/5; AAV2/6; AAV2/7; AAV2/8; AAV2/9; AAV2/DJ; AAV2/DJ8; AAV2/PHP.B; AAV2/PHP.eB; AAV2/PHP.S; AAV2/PHP.N; AAV2/PHP.V1; AAV2/retro; AAV2/rh10

Virus Type

Adeno-Associated Virus (AAV)

Applications

Neural Tracing

Research Areas

Neural Circuit Mapping

Promoter

mDLX

Properites

Fluorophore

tdTomato

Shipping

Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Handling Advice

The lentivirus is generally referred to as BSL-2. AAV is usually considered BSL-1, but may require BSL-2 handling depending on the insert.

Storage

Store at -80°C. Thaw just before use and keep on ice.

Quality Control

Creative Biolabs ensures high-quality viral vectors by optimizing and standardizing production plans and implementing strict quality control (QC). The specific QC analysis performed for each virus batch is different.

Titer: The precise titer of the sample will be reported on the test tube. The potency you see on this page is the guaranteed minimum potency.

Research Use Only

For research use only.

Publications

Publications (0)

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Scientific Resources

[Brochure]

Virus Vector Tools for Neural Cell Manipulation

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[Flyer]

Ready-to-Use AAV Biosensors

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[Knowledge Sharing]

Applications of AAV and LV in Nervous System Research

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Stand out for its specificity and robustness

AAV-mDLX-CRE-tdTomato vector provided excellent results in our neural tracing experiments. The specificity for mDLX-expressing neurons was impressive, and the fluorescent signal was clear and bright. We encountered no issues with transduction efficiency or expression levels. This tool has significantly enhanced our ability to map neural circuits accurately.

Excellent

Our lab has been using the AAV-mDLX-CRE-tdTomato virus for several months now

It has consistently delivered high-quality results. The tdTomato fluorescence allows for easy visualization of neural pathways, and the CRE-dependent expression ensures specificity. The virus was easy to handle and integrate into our existing protocols.

Excellent

A reliable and effective tool in our research

We were particularly impressed with the clarity and intensity of the tdTomato signal in our neural tracing studies using the AAV-mDLX-CRE-tdTomato. The virus exhibited high transduction efficiency, and the fluorescence was sustained over time, facilitating long-term studies.

Excellent

Which was exactly what we needed for our studies

The tdTomato fluorescence is robust and provides excellent contrast, making it easy to distinguish labeled neurons. The product was well-packaged, and the technical support provided was very helpful in optimizing our experimental protocols.

Excellent

The product was delivered promptly and in excellent condition, ready for immediate use

Our experience with AAV-mDLX-CRE-tdTomato has been very positive. The fluorescence is easily detectable, and the virus was easy to work with. This product has enhanced our neural tracing capabilities significantly.

Q&As

Could you provide a recommended injection protocol for using this vector in mice?

For mice, we recommend injecting the AAV-mDLX-CRE-tdTomato directly into the target brain region using a stereotaxic apparatus. The optimal viral titer is typically around 10¹² to 10¹³ GC/ml. Ensure the injection volume does not exceed 1-2 µL per site to minimize tissue damage. After injection, allow 2-4 weeks for expression before conducting further analysis.

Does the AAV-mDLX-CRE-tdTomato vector cause any cytotoxicity in neurons?

The AAV-mDLX-CRE-tdTomato is generally considered safe and exhibits minimal cytotoxicity. AAV vectors are widely used in neuroscience due to their low immunogenicity and ability to transduce neurons without significant adverse effects. However, as with any viral vector, it is essential to optimize viral load and injection parameters to minimize potential cytotoxicity.

What benefits does the tdTomato marker provide in neural tracing experiments?

The tdTomato fluorescent protein marker is a robust and stable reporter that emits bright red fluorescence, making it highly visible under a fluorescence microscope. This allows for detailed visualization of neuronal structures and processes, facilitating the tracing of neural pathways. Its strong signal enables precise anatomical mapping, critical for understanding complex neural networks.