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Creative Biolabs

AAV2/2Retro-hSyn-GCaMP6f-WPRE-pA [Calcium Sensor]

[CAT#: NTA-2012-ZP299]

Tracer Type:
Calcium Sensor
Applications:
Calcium imaging

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Description

The hSyn promoter, also called hSyn1 promoter, is cloned from the promoter region of human synapsin1 (Synapsin1), is about 0.5kb in length, and expresses specifically in neurons.

GCaMP6f, a type of GCaMP protein, is composed of three structures: cpGFP protein, CaM (calmodulin), and M13 peptide. When calcium ions are present, CaM and M13 combine to cause the conformation of cpGFP protein and enhance the fluorescence signal. . It is used to indicate the Ca signal activity in cells in experiments, and is also called GeCI (genetically encoded calcium indicator). GCaMP6 is a new generation of GCaMP protein, and its calcium indicator function reaches or exceeds the commonly used chemical calcium indicator. It has three commonly used subtypes, 6s, 6f and 6m. Among them, the binding force for calcium ions is 6s>6m>6f, so 6f dissociates fastest and is suitable for high-frequency signal indication.

Tracer Type

Calcium Sensor

Functional Gene

GCaMP6f

Serotype

AAV Retro

Virus Type

Adeno-Associated Virus (AAV)

Applications

Calcium imaging

Research Areas

Neural Circuit Mapping

Promoter

Syn
Properites

Color

Green
Data

Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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