Product Overview

Description

fDIO, or fDiO (Flpcontroled Double-floxed inverse Orientation), is an element that cooperates with Flp to control the expression of the target gene. It generally consists of two pairs of mutually incompatible Frt sequences. The characteristic sequence is Frt-interval 1-Frt5-GOIr- Ftrr-Interval 2-Frt5r. When Flp is present, any pair of Frt sequences may recombine. Because each pair of Frt is in the opposite direction, recombination will cause the sequence between them to be reversed. For example, Frt recombines first to form Frt-GOI- Frt5r-interval 1r-Frtr-interval 2-Frt5r, then the direction of the two Frt5 is changed from the previous opposite to the same, the second recombination between them will make Ftrr missing, forming Ftr-GOI-Frt5r, due to Frt No recombination can occur between Frt5 and Frt5, and the position of the target gene is fixed. Frt5 reorganizes first, and the result is the same. Usually we insert the target gene so that its sequence is opposite to the promoter, that is, it is not expressed at the beginning, and the target gene begins to be expressed after Cre acts.

The hEF1a promoter, cloned from the promoter region of human translation elongation factor 1A1 (eukaryotic translation elongation factor 1 alpha 1 (EEF1A1)), is 1.3 kb in length, expresses constitutively, and has no cell tissue specificity.

GtACR1, Guillardia thetaanion channel rhodopsins 1, is an anion channel protein that is opened under light stimulation and can permeate negative ions such as Cl- to inhibit neuronal cell activity. Its channel properties determine that it is more efficient than the commonly used NpHR. Compared with the artificially modified chloride channel fastChloC, the required light intensity is greatly reduced. Compared with slow ChloC, the reaction time of the GtACR1 channel is very high. fast. At the same time, the light inactivation effect is low. The optimal wavelength for GtACR1 activation is 515nm.