AAV2/9-hSyn-Flpo-EGFP-WPRE-pA

Product Overview

Description

Flp (flippase) is a commonly used site-specific DNA recombinase, cloned from Saccharomyces cerevisiae, composed of 423 amino acids, and the site of Flp is FRT. Some Frt mutants can also be recognized by Flp, including F3, etc. Usually these mutants can only recombine with their own sequence. On the same DNA molecule, depending on the direction of the two FRT sequences that undergo recombination, the sequence between the two sequences will be deleted or reversed.

The hSyn promoter, also called hSyn1 promoter, is cloned from the promoter region of human synapsin1 (Synapsin1), is about 0.5kb in length, and expresses specifically in neurons.

Tracer Type

Recombinase

Serotype

AAV9

Virus Type

Adeno-Associated Virus (AAV)

Applications

Recombinase

Research Areas

Neural Circuit Mapping

Promoter

Syn

Expression

Flp-dependent

Properites

Fluorophore

EGFP

Data

Product Pictures

Publications

Publications (0)

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Scientific Resources

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

This vector has significantly enhanced the precision of my experiments

The efficiency of Flp recombinase expression is outstanding, and the EGFP reporter provides clear, bright fluorescence for imaging. This vector has significantly enhanced the precision of my experiments, allowing for more accurate mapping of neural pathways. I highly recommend it for anyone involved in neurobiology research.

Excellent

The AAV2/9 serotype is perfect for CNS applications

This construct with hSyn promoter drives robust expression in neurons. The Flpo recombinase system worked seamlessly in my transgenic mouse models, and the EGFP fluorescence was strong and persistent. This product has become a staple in our lab for conditional gene manipulation studies.

Excellent

I was impressed with the packaging quality and titer of the vector

It arrived promptly and was ready to use. In my experiments, the vector showed high transduction efficiency in hippocampal neurons, and the EGFP signal was bright enough for confocal microscopy. This tool has been invaluable for my research on synaptic plasticity.

Excellent

Streamlined our workflow for gene editing in neuronal cultures

The Flpo/Frt system is highly efficient, and the EGFP reporter allows for easy visualization of transduced cells. The vector's performance exceeded my expectations, providing consistent and reproducible results across multiple experiments.

Q&As

How does the Flpo recombinase function in this vector, and what are its benefits for neuroscience studies?

The Flpo recombinase in this vector allows for site-specific recombination of DNA sequences flanked by FRT (FLP Recognition Target) sites. This technology enables precise manipulation of genes by allowing researchers to activate or deactivate specific genes in targeted neurons. The benefits for neuroscience studies include the ability to study gene function in a spatially and temporally controlled manner, which is crucial for understanding complex neural processes and disease mechanisms.

How stable is the expression of the Flpo recombinase and EGFP over time?

The expression of Flpo recombinase and EGFP is generally stable over time due to the inclusion of the WPRE element, which enhances transcript stability and translation efficiency. However, the stability of expression can vary depending on factors such as the target tissue, the age of the animal, and the presence of immune responses. Regular monitoring and validation of expression levels are recommended to ensure consistent results in experiments.

How can I confirm successful transduction and expression of the Flpo recombinase and EGFP?

Successful transduction and expression can be confirmed using several methods, including fluorescence microscopy to visualize EGFP expression in tissue sections or live cells. Additionally, you can perform PCR or Western blotting to detect the presence of the Flpo recombinase and assess its activity. Histological staining or immunohistochemistry can also be used to evaluate the specific expression in targeted neuronal populations.