Product
Alzheimer's 3Dish™ PSEN1-RFP Lentivirus
[CAT#: NCL-2108-P006]
The PSEN1-RFP lentivirus can be used to generate 3D cell culture models of Alzheimer's Disease. The high-titer polycistronic lentiviral particles contain the PSEN1 and TagRFP2 proteins and can be used to infect human neural stem cells at high efficiency.
- Applications:
- Cell Culture
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Product Overview
Description
We transfected NeuCELL™ VM human neural stem cells with lentiviruses expressing PSEN1 with the E9 mutation (PSEN1(E9)) and fluorescent proteins as reporters for viral infection. The highest transgene-expressing cells were sought out using FACS, and the cells were then encapsulated in a 3D Matrigel culture medium. Aggregation of AB was noticed during a differentiation period of roughly six weeks. After 10-14 weeks, it was easy to see a robust deposition of phosphorylated tau along neurofibrillary tangles.
Applications
Cell Culture
Relevant Diseases
Alzheimer's Disease
Properties
Storage
Lentivirus is stable for at least 6 months when stored at -80°C. After first thaw, place immediately on ice and store in working aliquots to avoid further freeze thaws. Avoid freeze thaws as this will result in a decrease in the virus titer.
Research Use Only
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Quality Control
The packaged lentivector constructs are provided as frozen VSV-G pseudotyped viral particles. The titer (ifus/ml) is defined by Lentiviral Titer Kit in HT1080 cells transduced by the packaged constructs.
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
