Bone Marrow Mononuclear Cell-Derived iPS Cell Line (CD34⁺, ApoE3, Episomal) (Human)

Apolipoprotein (apo) E is a polymorphic protein with 299 amino acids and has important and diverse functions in neurobiology. ApoE can distribute lipids between cells in the central nervous system to achieve normal lipid homeostasis and participate in neuronal repair and remodeling. ApoE includes three main human isoforms, apoE2, apoE3 and apoE4, and they differ in their ability to complete these tasks. Human ApoE3 and ApoE4 differ from each other only at one amino acid residue at position 112. The common isoform ApoE3 has Cys112, while ApoE4 has Arg112.

The footprint-free human iPS (induced pluripotent stem) cell line (cat # NCL200546ZP) carries APOE-ε4 in both alleles, and was used for generating an isogenic iPSC line (cat # NCL200547ZP) with APOE-ε3 in both alleles by changing Arg112 to Cys112. Sequencing results confirmed that the iPSC line had stable homozygous transformation with Cys112 (TGC) of Arg112 (CGC) in APOE gene. In addition, another syngeneic ApoE deficient (knockout iPSC strain (Cat. No. NCL200548ZP) also comes from the same iPS16. The morphology of this human iPS cell line is the same as that of human ES cells. These cells also express the pluripotency marker TRA-1-60 , SSEA-3 and Oct4, and show strong endogenous alkaline phosphatase activity.

In summary, the human iPS (induced pluripotent stem) cell lines (catalog numbers NCL200546ZP, NCL200547ZP and NCL200548ZP) are syngeneic. Lines NCL200547ZP (ApoE3) and NCL200548ZP (ApoE-KO) are derived from line NCL200546ZP (ApoE4). These cell lines have the same genetic background and are good tools for studying AD-related diseases in neurons specifically induced by ApoE4.

iPSC

Cell Assay

Neurological Disorders

TRA-1-60/ Oct4

Human

2x10e5 cells/vial

Frozen Cryovials/Live Culture

Live cells are in mid log-phase growth.

Human bone marrow mononuclear cell

>95%

>90%

Cells are negative for mycoplasma contamination.

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Dry ice

Liquid nitrogen

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

For research use only, not for diagnostic or therapeutic use.

Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.

Human iPS cells were cultured in mTeSR1 medium without feeder layers. After recovery from cryopreservation, each batch of human iPS cells was tested for growth and viability. In addition, each batch was tested for TRA-1-60 and Oct4 expression and alkaline phosphatase activity.