Bone Marrow Mononuclear Cell-Derived iPS Cell Line (CD34⁺, ApoE4, Episomal) (Human)
A footprint-free human iPS (induced pluripotent stem) cell line was derived from human bone marrow CD34+ mononuclear cells by ectopic expression of OCT4, SOX2, KLF4, c-MYC and Lin28 genes using episomal plasmids.
The footprint-free human iPS (induced pluripotent stem) cell line (cat # NCL200546ZP) carries APOE-ε4 in both alleles, and was used for generating an isogenic iPSC line (cat # NCL200547ZP) with APOE-ε3 in both alleles by changing Arg112 to Cys112. Sequencing results confirmed that the iPSC line had stable homozygous transformation with Cys112 (TGC) of Arg112 (CGC) in APOE gene. In addition, another syngeneic ApoE deficient (knockout iPSC strain (Cat. No. NCL200548ZP) also comes from the same iPS16. The morphology of this human iPS cell line is the same as that of human ES cells. These cells also express the pluripotency marker TRA-1-60 , SSEA-3 and Oct4, and show strong endogenous alkaline phosphatase activity.
In summary, the human iPS (induced pluripotent stem) cell lines (catalog numbers NCL200546ZP, NCL200547ZP and NCL200548ZP) are syngeneic. Lines NCL200547ZP (ApoE3) and NCL200548ZP (ApoE-KO) are derived from line NCL200546ZP (ApoE4). These cell lines have the same genetic background and are good tools for studying AD-related diseases in neurons specifically induced by ApoE4.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
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