Calcium Flux and cAMP Assay Service

Calcium flux & cAMP Assay is a crucial technology in cell signaling research, enabling the analysis of dynamic changes in intracellular calcium ions (Ca²⁺) and cyclic adenosine monophosphate (cAMP).

Creative Biolabs is pleased to announce the launch of our cutting-edge Calcium flux & cAMP Assay service, which provides an effective solution for studying intracellular signaling pathways. To learn more about our products and services, submit a project inquiry, or request pricing, please contact us at your earliest convenience.

Calcium Flux Assay

Calcium flux assays are primarily used to study changes in intracellular Ca²⁺ concentrations. Calcium ions play a key role as second messengers in many biological processes. To illuminate the role of these ions, calcium flux assays typically use fluorescent probes that change their fluorescence properties upon binding to Ca²⁺, allowing changes in intracellular Ca²⁺ concentrations to be monitored by techniques such as fluorescence microscopy and flow cytometry. Calcium flux assays are often used to screen drugs for G protein-coupled receptors (GPCRs) and calcium channels.

Fig.1 Calcium flux in cells mediated by NMDARs.¹ A) The sensitivity of NMDAR-mediated calcium flux in MDL105,519-protected cells to glycine. B) The sensitivity of NMDAR-mediated calcium flux in CGP070667-protected cells to glutamate.

cAMP Assay

The cAMP flux assay focuses on changes in intracellular cAMP levels. cAMP is an important secondary messenger that regulates a variety of cellular functions. cAMP flux assay methods include ELISA, bioluminescence, and high-throughput homogeneous ELISA, which provide high sensitivity and high-throughput detection. In addition, live cell imaging techniques are used to monitor changes in cAMP levels in real time, such as the use of FRET sensors for dynamic measurement of cAMP levels.

The combination of calcium flux and cAMP assays offers a more comprehensive approach to the study of signaling. For example, in certain cell types, there is a sophisticated interplay between calcium and cAMP signaling that may influence the cell's response to diverse stimuli.

Applications of Calcium Flux & cAMP Assay

• Drug Discovery and Screening
• GPCR Pharmacology
• Ion Channel Function and Modulation
• Toxicity and Safety Assessment
• Neuronal Excitability and Synaptic Plasticity
• Neurotransmitter Research

At Creative Biolabs, we offer a range of options for customizing calcium flux & cAMP assay to meet your specific requirements. In addition, we provide high-quality materials, data, and insights to enhance the success of your project. Please contact us to discuss your project plans in more detail.

Reference

1. Guo, Hongqiu, et al. "A NMDA-receptor calcium influx assay sensitive to stimulation by glutamate and glycine/D-serine." Scientific reports 7.1 (2017): 11608. Distributed under Open Access license CC BY 4.0, without modification.