Service

Calcium Flux & cAMP Assay

Creative Biolabs is a renowned company at the forefront of neuroscience research and services. We are thrilled to introduce our Calcium Flux & cAMP Assay service, a cutting-edge solution for investigating intracellular signaling pathways.

Calcitonin Receptor Assay

Calcitonin receptors play a crucial role in calcium homeostasis and are involved in various physiological processes, including bone remodeling, calcium regulation, and neuronal function. Our Calcium Flux & cAMP Assay service includes a specific focus on the Calcitonin Receptor Assay, which allows for measuring both cAMP & Calcium concentration changes in the cytosol upon activation with calcitonin.

By using fluorescent Calcitonin Receptor Cell Line, we can monitor changes in intracellular calcium levels upon stimulation of the calcitonin receptor. These assays provide real-time, quantitative measurements of calcium flux, enabling the assessment of receptor functionality and pharmacological modulation.

High-throughput Calcium Flux Assays (FLIPR Assay)

At Creative Biolabs, we offer the FLIPR (Fluorometric Imaging Plate Reader) Assay, a cutting-edge technology that revolutionizes high-throughput screening in drug discovery and biomedical research. The FLIPR Assay enables rapid and accurate measurement of cellular responses, particularly changes in intracellular calcium levels, upon stimulation by various compounds or conditions. By measuring calcium flux in real-time, the FLIPR Assay provides valuable insights into the activity of cellular pathways and the effects of potential drugs or compounds. This approach allows for the screening of compound libraries, identification of lead candidates, and optimization of drug candidates for calcium-related targets.

The workflow of high-throughput Calcium Flux assays involves the following steps:

Sample Preparation

Cells expressing the target receptor or cells of interest are cultured and prepared for the assay. These cells can be derived from various sources, including primary cells, immortalized cell lines, or stem cell-derived cell models.

Assay Setup

The prepared cells are loaded with calcium-sensitive dyes or transfected with genetically encoded calcium indicators. The cells are then distributed into multi-well plates suitable for high-throughput screening.

Stimulation and Detection

The cells are stimulated with specific ligands or compounds of interest, and changes in intracellular calcium levels are measured using fluorescence-based imaging or plate readers. Real-time kinetic data can be obtained to assess calcium flux dynamics.

Data Analysis

The acquired fluorescence data are analyzed using dedicated software tools. Calcium peaks, response kinetics, and other relevant parameters can be extracted to evaluate the activation or inhibition of calcium signaling pathways.

Applications of Calcium Flux & cAMP Assay

High-throughput Calcium Flux assays offer several advantages, including increased assay speed, reduced sample consumption, and the ability to screen large compound libraries. These assays are particularly useful in drug discovery and screening applications.

Drug Discovery and Screening
GPCR Pharmacology
Ion Channel Function and Modulation
Toxicity and Safety Assessment
Neuronal Excitability and Synaptic Plasticity
Neurotransmitter Research

Creative Biolabs' Calcium Flux & cAMP Assay service provides a powerful tool for investigating calcium flux and cAMP dynamics in diverse biological contexts. With applications ranging from GPCR signaling studies to neuronal excitability and endocrine disorders, our service facilitates breakthroughs in neuroscience research and therapeutic development.

Please feel free to contact us for more about our GPCR related services.

For Research Use Only. Not For Clinical Use.