Calcium Imaging Tools
Calcium imaging refers to the use of calcium ion indicators to monitor the concentration of calcium ions in cells, tissues or culture media. This technique is widely used to study calcium signaling in neuronal activity in vivo and in vitro.
Calcium ion is an essential intracellular messenger in mammalian neurons. In the resting state, the intracellular calcium ion concentration of most neurons is about 50-100 nM, which can increase 10-100 times during electrical activity. As an intracellular signal, calcium ions can trigger responses, such as altering gene expression and the release of neurotransmitters in synaptic vesicles. Because there are ion pumps in cells that selectively transport these ions under various signal stimuli, the intracellular calcium concentration is highly dynamic. Calcium imaging technology uses the advantages of calcium ion flow and special fluorescent dyes or protein fluorescent probes (calcium indicator) to directly visualize calcium signals on living nerve cells, so as to achieve the purpose of monitoring neuronal activity.
In addtion to providing neural viral vector tracers, Creative Biolabs also offers relevant AAV packaging services. For the pre-made AAV tools, please contact us for more details.