Clearing Kits

When studying the brain in an anatomical setting, immunohistochemical approaches often involve imaging one tiny slice at a time. Using traditional methods to obtain micron-thick sections, fix, stain, image, and finally stitch all the sections together is a time-consuming process, especially for large pieces of tissue. Tissue clearing methods allow researchers to "see" the intact mouse brain, identify specific cells, and eliminate all the slicing and stitching to see deep into the tissue.

Applications

Which scientific questions may these methods of tissue cleaning help answer? These methods enable the resolution of issues like:

• Identifying the destiny of cells within a certain area.
• Identifying the localization of particular cell populations to unveil anatomical circuits.
• Examining intricate synaptic architecture in an intact brain while avoiding the introduction of artifacts.
• These inquiries apply to comprehending neurodegenerative diseases like AD as well as developmental and basic neuroscience.

Case Study

AD development is driven by the dynamic creation, progression, and interaction of pathological hallmarks such as amyloid-β (Aβ) plaques and neurofibrillary tangles (NFTs). The use of tissue clearing techniques can answer much more queries than 2D systems. For example: What types of cells and/or structures do Aβ plaques interact with? Does the disease start in a specific area of the brain, possibly with NFTs in a subregion, then spread throughout the brain? Does it vary depending on the extent of the disease or the region of the brain being studied?

The researchers studied the entire brain of an AD mouse model stained for β-Amyloid. These questions can be further addressed. Researchers used tissue clearing combined with antibody staining to reveal Aβ plaques throughout the brain. Whole-brain tissue clearance was employed to observe a reduction in Aβ plaques generated by gamma frequency stimulation in the context of the whole brain of mice. This is an encouraging breakthrough towards future AD treatments.

Confocal reconstruction of the brain of a mouse model of AD, which was cleared and stained for amyloid.¹

Clear3D™ Clearing Kits from Creative Biolabs are the optimal solution for next-generation 3D histology across a spectrum of tissues. They are the preferred choice for researchers seeking a straightforward approach to achieving expert-level tissue clearing.

If the product you're looking for isn't mentioned, or if you'd like to purchase one of our clearing kits, please contact us. Our professionals will aid you in picking the appropriate product for your unique application and locating the best product for the task.

Reference

1. Martorell, Anthony J., et al. "Multi-sensory gamma stimulation ameliorates Alzheimer's-associated pathology and improves cognition." Cell 177.2 (2019): 256-271.