Creative Biolabs

Labeled Dextran Conjugates

Labeled Dextran Conjugates

Dextran is a natural synthetic polysaccharide with good water solubility, low toxicity and different molecular weight. They have been successfully used as long-term tracers for living cells. Our dextran is purified by size chromatography to remove unbound molecules and ensure proper cell biology analysis applications. Dextran is functionalized with various groups, dyes and other biomolecules.

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Dextrans Features

Dextrans is a hydrophilic polysaccharide synthesized by Leuconostoc bacteria. They have a high molecular weight and have various sizes after fractionation. Dextran has good water solubility, low toxicity and relatively inertness. Their α-1,6-polyglucose bonds are resistant to cleavage by most endogenous cellular glycosidases. These properties enable Dextrans to be used as an effective water-soluble carrier for dyes, indicators and reactive groups in various applications, especially as a long-term tracer for living cells. Dextran conjugates with a variety of molecular weights (3 000, 10 000, 40 000, 70 000, 500 000) and labeled with a wide range of substituents can be used.

Advantages of Dextran for neuron staining:

  • 3,000 and 10,000 MW
  • Direct fluorescence or secondary detection
  • Loading by microinjection or iontophoresis
  • Repairable and light stabilized options

Applications

Fluorescent and biotinylated dextran are commonly used to track neuronal projections. Dextran conjugates with a molecular weight of up to 70 000 Daltons are widely used to track neuronal projections. The 3 000 MW dextran conjugate penetrates the peripheral neuronal processes better than the high MW dextran and diffuses faster. In contrast, 10,000 MW dextran remains in the cell and does not cross gap junctions. They distribute slowly, but the cells retain longer. The 10,000 MW AlexaFluor® conjugate is generally brighter and more light resistant. Most conjugates are lysine immobilized and can be used in combination with antibody labels. The labeled dextran can be effectively used as an antegrade or retrograde tracer, depending on the research method and tissue type used.

  3,000 MW Dextran 3,000 MW Dextran, Fluorophore, Biotin 10,000 MW Dextran, Alexa Fluor® 10,000 MW Dextran, Fluorophore, Biotin
Readout Direct fluorescence imaging of cells Direct fluorescence imaging of cells; biotins easily accept secondary detection to amplify weak signals Direct fluorescence imaging of cells Direct fluorescence imaging of cells; biotins easily accept secondary detection to amplify weak signals
Carrier Dextran, 3,000 MW Dextran, biotin, 3,000 MW Dextran, 10,000 MW Dextran, biotin 10,000 MW
Tissue sections Yes Yes
In vivo neurons Yes Yes
Fixed tissue sections No No
Cultured cells Yes Yes
Cell loading Microinjection Microinjection
Staining mode Endogenous Endogenous
Best for Single cells Single cells
Fixable   Lysine-fixable
Transport Bidirectional Bidirectional
Synaptic transfer No No
Gap junction transfer Yes No
Cat. No. Product Name Standard filter set(s) Fluorophore Ex/Em (nm)
NTA-2011-ZP90 Dextran, Biotin-Conjugated, 3,000 MW [Lysine-Fixable] [Lysine-Fixable] NA Biotin NA
NTA-2011-ZP91 Dextran, Fluorescein and Biotin-Conjugated, 3,000 MW [Lysine-Fixable] FITC Fluorescein 494/518
NTA-2011-ZP92 Dextran, Tetramethylrhodamine and Biotin-Conjugated, 3,000 MW [Lysine-Fixable] TAMRA TAMRA 555/580
NTA-2011-ZP93 Dextran, Fluorescein-Conjugated, 3,000 MW [Lysine-Fixable] FITC Fluorescein 494/521
NTA-2011-ZP94 Dextran, Tetramethylrhodamine-Conjugated, 3,000 MW [Lysine-Fixable] TAMRA TAMRA 555/580
NTA-2011-ZP95 Dextran, Texas Red®-Conjugated, 3,000 MW [Lysine-Fixable] Texas Red® Texas Red® 595/615
NTA-2011-ZP96 Dextran, Rhodamine Green-Conjugated, 3,000 MW [Non-Fixable] FITC Rhodamine Green 502/527
NTA-2011-ZP97 Dextran, Tetramethylrhodamine-Conjugated, 3,000 MW [Non-Fixable] TAMRA TAMRA 555/580
NTA-2011-ZP98 Dextran, Texas Red®-Conjugated, 3,000 MW [Non-Fixable] Texas Red® Texas Red® 595/615
Cat. No. Product Name Standard filter set(s) Fluorophore Ex/Em (nm)
NTA-2011-ZP99 Dextran, Alexa Fluor® 488-Conjugated, 10,000 MW FITC Alexa Fluor® 488 495/519
NTA-2011-ZP100 Dextran, Alexa Fluor® 546-Conjugated, 10,000 MW TRITC Alexa Fluor® 546 556/573
NTA-2011-ZP101 Dextran, Alexa Fluor® 555-Conjugated, 10,000 MW Alexa Fluor® 555 555/565
NTA-2011-ZP102 Dextran, Alexa Fluor® 568-Conjugated, 10,000 MW Rhodamine Alexa Fluor® 568 578/603
NTA-2011-ZP103 Dextran, Alexa Fluor® 594-Conjugated, 10,000 MW Texas Red® Alexa Fluor® 594 590/617
NTA-2011-ZP104 Dextran, Alexa Fluor® 647-Conjugated, 10,000 MW Cy®5 Alexa Fluor® 647 650/668
NTA-2011-ZP105 Dextran, Alexa Fluor® 680-Conjugated, 10,000 MW Cy®5.5 Alexa Fluor® 680 679/702
NTA-2011-ZP106 Dextran, Tetramethylrhodamine and Biotin-Conjugated, 10,000 MW TAMRA TAMRA 555/580
NTA-2011-ZP107 Dextran, Fluorescein and Biotin-Conjugated, 10,000 MW FITC Fluorescein 494/518
Cat. No. Product Name Ex/Em (nm) MW g.mol-1
NTA-2011-ZP108 Dextran-amine   3 000
5 000
10 000
40 000
70 000
500 000
2 000 000
NTA-2011-ZP109 Dextran-Blue (Cibacron blue F3GA) 621.5 / - 5 000
10 000
70 000
110 000
500 000
2 000 000
NTA-2011-ZP110 Dextran–FITC 494/518 3 000
4 000
10 000
20 000
40 000
70 000
500 000
NTA-2011-ZP111 Dextran-FITC, Lysine Fixable 494/518 3 000
4 000
10 000
20 000
40 000
70 000
500 000
NTA-2011-ZP112 Dextran–biotin   3 000
10 000
40 000
70 000
500 000
NTA-2011-ZP113 Dextran-biotin, Lysine Fixable   3 000
10 000
40 000
70 000
500 000
NTA-2011-ZP114 Dextran–Rhodamine B 555/580 3 000
10 000
40 000
70 000
500 000
NTA-2011-ZP115 Dextran-TMR, Lysine Fixable 555/580 3 000
10 000
40 000
70 000
500 000
NTA-2011-ZP116 Dextran-TRITC 555/580 4 000
NTA-2011-ZP117 Dextran-CYanine5 580/675 3 000
10 000
40 000
70 000
500 000
NTA-2011-ZP118 Dextran-CYanine5.5 663/690 3 000
10 000
40 000
70 000
500 000
NTA-2011-ZP119 Dextran-CYanine7 743/767 8 000

In addition to reactive dye formulations, we also provide dyes conjugated with a variety of antibodies, peptides, proteins, tracers and amplification substrates to optimize cell labeling and detection. Please contact us for more details.

For Research Use Only. Not For Clinical Use.
Neural Tracers
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