Stain Kits
Researchers employ histological staining to analyze how cells, structures, and molecules are arranged in tissues and organs. In this technique, the tissue of interest is preserved in a chemical fixative and sliced, or cut into very thin sections. Various staining techniques are then used to provide contrast to these seemingly uniform sections. In neuroanatomy, histological techniques are often used to observe and study the tissues of the nervous system.
Creative Biolabs offers a diverse assortment of neuroscience staining kits based on our extensive experience. The NeuStain™ kits from our neuroscience experts will help you get high quality images in just a few simple steps.
Nissl Stain
The Nissl stain was introduced in the late 19th century by the German neurologist Franz Nissl. Nissl used a class of basic dyes that stained the nuclei of all cells and the clumps of material around the nuclei of neurons, called Nissl bodies. Nissl stain has the following advantages: it can distinguish neurons from glial cells; it allows histologists to study the arrangement or cytoarchitecture.
Fig.1 Nissl can stain the nuclei of all cells.1
Golgi Stain
However, not all problems can be solved by Nissl stain. Italian histologist Camillo Golgi invented a new way to learn more about neurons. In 1873, Golgi discovered that soaking brain tissue in a solution of silver chromate would cause a small portion of the neurons to turn completely black. This method is now called the Golgi stain. This revealed that the cell body of the neuron (i.e., the area around the neuron nucleus shown by Nissl staining) is only a small part of the entire neuron structure.
Fig.2 Golgi-Cox staining for brain tissue.2
NeuStain™
With the motto "The most important achievements in brain research come from staining", neurohistology is still an active field of neuroscience today.
NeuStain™ kits help you get staining results quickly, provide you with high-quality staining images, flexible protocols, and are compatible with a wide range of equipment.
In addition to providing high-quality stain kits, we also provide one-stop staining services. Creative Biolabs' histology and immunohistochemistry services are custom developed and fully optimized to meet your needs in neuroscience preclinical research.
If the product you require is not listed, or if you are interested in our custom services, please contact us. Our experts will assist you in selecting the most suitable product for your specific application and help you find the ideal product for the task.
References
- Lopes, M. Beatriz S. "Diagnostic histochemistry in neuropathology." Seminars in Diagnostic Pathology. Vol. 36. No. 1. WB Saunders, 2019.
- Zaqout, Sami, and Angela M. Kaindl. "Golgi-Cox staining step by step." Frontiers in neuroanatomy 10 (2016): 38.
Carboxylated and sulfated mucopolysaccharides and glycoprotein
Basement membrane
Mucin
Collagen, muscle tissue, reticular fibers, mucins and fibrin
Detection of mitochondrial function
Copper deposits
Argentaffin granules and melanin
Elastic fibers
Reticular fibers
Collagen fibers
Collagen and muscle
Nerve fibers, neurites and neurofibrillary tangles
Connective fibers
Mast cells
Lipid and fat staining on frozen sections
Amyloid deposits
Nissl body in the cytoplasm of neurons
Ferric iron in tissue sections
Calcium on tissue sections
Lipid and fat staining on frozen sections
Both acidic and neutral mucins as well as mixtures of acidic and neutral mucins
Glycogen, mucin and fungi
Tissue mucosubstances
General morphology of tissues and cells
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