CD1 Brain Cortex Neurons [Mouse]
Cryopreserved ampule of Mouse CD1 Cortex Neurons containing ≥ 4 million cells
Each vial cortex cell contains approximately 4 million living cells. Using the recommended seeding density and medium, seed it into 10 or more wells of a 24-well plate and 50 or more wells of a 96-well plate. After about 4 days of culture, the cells will form a network of neurites, and by day 7, fragmentation will be minimized.
Recommended medium: CocKit™ Primary Neuron Growth Medium (NCC20-9PZ10)
• Positive immunofluorescence staining for glial fibrillary acid protein (GFAP)
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
• HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
• A Certificate ofAnalysis is provided for each cell lot purchased