Creative Biolabs

Cortical Brain Tissue-Derived ImtCell™ Immortalized Neural Progenitor Cell Line (Human)

[CAT#: NCL200501ZP]

An immortalized human neural progenitor cell line can easily differentiate into neurons and glial cells.

Species:
Human
Applications:
Cell Assay
Cell Types:
Neural Stem Cell; Neural Progenitor Cell

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Description
The immortalized human neural progenitor cell line has the ability to easily differentiate into neurons and glial cells. Derived from the cortical area of the human fetal brain. Immortalized by retrovirus transduction of c-myc oncogene, the cell line grows rapidly in the form of a single layer of laminin with a doubling time of 20-30 hours. Karyotype analysis showed that even after long passages (> 45 passages), the cell lines still retain normal diploid karyotypes in culture. It can be used for various research applications, such as neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor function.
Cell Types
Neural Stem Cell; Neural Progenitor Cell
Applications
Cell Assay
Relevant Diseases
Alzheimer's Disease; Neurological Disorders
Species
Human
Size
Each vial contains ≥ 1X10e6 viable cells.
Form
Frozen Cryovials/Live Culture
Cell State
Live cells are in mid log-phase growth.
Tissue Source
Human cortical brain tissue
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Each batch of cells has passed the high-level expression of Nestin and Sox 2 as well as its self-renewal and multi-lineage differentiation ability. The cells also showed normal karyotypes, and mycoplasma tests were negative by chromosome spread assessment. The blood of pregnant women has been screened for HIV, HTLV, hepatitis B and C.
For Research Use Only. Not For Clinical Use.
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