Cortisol EIA Kit, Species Independent

Description

The Cortisol EIA kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma and tissue culture media samples. This kit measures total cortisol in extracted samples and in serum and plasma and free cortisol in saliva and urine. A cortisol standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol to each well. After an 1 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the cortisol in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Species Reactivity

Wide Species

Applications

Competitive EIA (Enzyme Immune Assay)

Relevant Diseases

Mental Disorders

Sensitivity

17.3 pg/ml

Platform

Microplate

Detection Method

Colorimetric Assay

Sample Type

Dried Fecal Samples, EDTA Plasma, Heparin Plasma, Saliva, Serum, Tissue Culture Media, Urine

Assay Type

EIA Kits

Assay Time

90 Minutes

Precision

Intra Assay Precision: Three human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Cortisol concentrations were: Sample 1- 1174.3 pg/mL, 6% CV Sample 2- 475.9 pg/mL, 5.6% CV Sample 3- 177.4 pg/mL, 14.7% CV Inter Assay Precision: Three human samples were diluted with Assay Buffer and run in duplicates in ten assays run over multiple days by four operators. The mean and precision of the calculated Cortisol concentrations were: Sample 1- 1188.1 pg/mL, 7.2% CV Sample 2- 508.7 pg/mL, 6.3% CV Sample 3- 199.7 pg/mL, 10.9% CV

Number Of Samples

40 samples in duplicate

Linear Range

100 - 3200 pg/ml

Components

Clear Microtitre 96 well Plate 1 Plate
Cortisol Standard 125 µl
StressXpress® Cortisol Antibody 3 ml
StressXpress® Cortisol Conjugate 3 ml
Assay Buffer 50 ml
Dissociation Reagent 1 ml
Wash Buffer Concentrate 50 ml
TMB Substrate 11 ml
Stop Solution 10 ml
Plate Sealer 1 Each

Shipping

Blue Ice

Storage

4°C

Research Use Only

For research use only

Target

Cortisol

Official Name

Cortisol

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