Product
DAPI
[CAT#: NE-200601P-CD]
Blue fluorescent DNA stain. Nuclear counterstain.
- Type:
- DNA / RNA Cell Dyes
- Applications:
- Cell Imaging; IF
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Lot Number
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Lot Number
Product Overview
Description
DAPI is a blue fluorescent DNA stain that can permeate cells at high concentrations.
DAPI firmly binds to the A-T-rich region in DNA to form a fluorescent complex. When combined with DNA, it preferentially stains ds-DNA and has a high quantum yield (φf = 0.92).
DAPI firmly binds to the A-T-rich region in DNA to form a fluorescent complex. When combined with DNA, it preferentially stains ds-DNA and has a high quantum yield (φf = 0.92).
Type
DNA / RNA Cell Dyes
Chemical Name
4',6-Diamidino-2-phenylindole dihydrochloride
Relevant Disease
Neurological Disorders
Applications
Cell Imaging; IF
Application Notes
DAPI is commonly used for nuclear and chromosome counterstaining.
It is preferred for staining dead cells. DAPI is less effective as a staining agent for living cells because it cannot effectively pass through membranes in living cells. Therefore, it may be necessary to use higher concentrations.
DAPI must penetrate and/or fix the cell to enter the cell and bind to DNA.
Because DAPI emits blue light, yellow fluorescence, green fluorescent molecules (such as fluorescein and GFP) or red fluorescent colors (such as Texas Red) there is almost no fluorescence overlap. Therefore, it is convenient for multiple determination.
DAPI has many applications, but it is usually used for cell imaging, cell counting, cell sorting (based on DNA content), apoptosis analysis and HCA (high content analysis).
It is preferred for staining dead cells. DAPI is less effective as a staining agent for living cells because it cannot effectively pass through membranes in living cells. Therefore, it may be necessary to use higher concentrations.
DAPI must penetrate and/or fix the cell to enter the cell and bind to DNA.
Because DAPI emits blue light, yellow fluorescence, green fluorescent molecules (such as fluorescein and GFP) or red fluorescent colors (such as Texas Red) there is almost no fluorescence overlap. Therefore, it is convenient for multiple determination.
DAPI has many applications, but it is usually used for cell imaging, cell counting, cell sorting (based on DNA content), apoptosis analysis and HCA (high content analysis).
Properties
Excitation
340 / 360nM (for ds-DNA)
Emission
488 / 460nM (for ds-DNA)
Molecular Weight
350.24
Chemical Formula
C16H15N5.2HCl
SMILES
C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl
Solubility
Soluble in water (10mg/ml, gentle warming), and in methanol
CAS NO.
28718-90-3
PubChem Substance ID
160166
Purity
>95%
Storage
-20°C
Shipping
Room Temperature
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
