Fluorescent Biomarkers in Neurons
Functional imaging has become one of the main ways to study the functions of different neurons, from subcellular studies of dendritic spines dynamics to whole-cell circuit-level imaging, to mesoscale, and chronic whole-brain imaging in small animals. Therefore, multiple tools are needed to study different aspects of neuronal signaling and function. Fluorescent molecular probe is a kind of non-genetically coded biosensor for directly labeling neurotransmitters developed in recent years. In the research of neurodegenerative diseases, fluorescent probe-mediated biomarker visualization technology has attracted more and more attention in the early diagnosis of neurodegenerative diseases.
Fluorescent Biomarkers in Neurons
- GABAA receptors
- Ionotropic glutamate (iGlu) receptors
- Fluorescent false neurotransmitters (FFNs)
- Quantum dots (QDs)
- Curcumin derivatives
- Fluorescent antibody
Ligand-based probes have also been used to screen potential drugs that may selectively bind to targeted receptors. GABAA receptor (GABA AR) can covalently label the fluorophore near the binding site, without affecting the binding of these compounds to the bimolecular fluorescence quenching and recovery, making the construction of fluorescent GABA AR ligand biosensors on the surface of living cells possible.
The probe used to display ionic glutamate (iGlu) receptors are synthesized based on fluorescent polyamine toxin analogs. The use of the natural product argiotoxin-636 (ArgTX-636) can block iGlu receptor ion channels. This probe is used to observe the natural NMDA receptor neurons in the hippocampus.
FFNs are optical probes for monoamine neurotransmission, usually composed of small fluorescent compounds and a recognition element, such as the ethylamine moiety. The research of FFNs mainly focuses on the synaptic control of neurotransmitter release: FFNs are loaded into vesicles to observe the presynaptic terminals and the release and uptake of neurotransmitters.
QDs are light-emitting semiconductor nanoparticles. Modification of QDs with antibodies or small molecule ligands can make them specifically bind to target proteins or cells to emit red light. QDs can be used to visualize dopamine receptors in vivo.
Due to the low fluorescence quantum yield of curcumin, the application of bioimaging was limited. Therefore, curcumin derivatives, CRANAD compounds, are prepared by converting the internal body into the corresponding Boron complex and substituting different aromatic groups for the curcumin acrylic group. Fluorescence is enhanced when the CRANAD compound binds to amyloid β (A+) plaques when tested in Alzheimer's disease (AD) samples.
Calbindin S100β, excitatory amino acid transporter (EAAT1+2), aldehyde dehydrogenase-1 family (ALDH1L1), sulfophosamine 101 (SR101) selectively stains astrocytes. These fluorescent antibodies selectively stain astrocytes, reactive astrocytes fluoresced in the red area when they are diseased.
Application of Fluorescent Probes in Degenerative Diseases
A+ plaques and neurofibrillary tangles (NFTs) are the two main pathological signs of AD. This prospect outlines the use of small fluorescent molecules to target AD biomarkers for pre-in vivo and in vivo imaging, providing significant advances in AD diagnostic technology.
The neurodegenerative process begins long before clinical symptoms become apparent and has progressed slowly and irreversibly. It is urgent to diagnose and distinguish the common and unique characteristics of different neurodegenerative diseases. Fluorescent marker-mediated probe technology has made a great contribution to the analysis of cognitive dysfunction and provides a deep understanding of the diagnosis of neurological diseases.
Creative Biolabs has a variety of reliable products in the diagnosis of neurodegenerative diseases. Our multiple fluorescein products can help you efficiently label the system and improve research efficiency and progress. Please feel free to contact us if you are interested or have any questions.

- Mouse Anti-Human α-Synuclein Phospho (Tyr39) (CBP3706) (Cat#: NAB201250LS)
- Mouse Anti-SCN5A Monoclonal Antibody (CBP708) (Cat#: NAB-0720-Z2720)
- iNeuMab™ Mouse Anti-SHANK3 Monoclonal Antibody (CBP929) (Cat#: NAB-0720-Z3477)
- iNeuMab™ Anti-F-Spondin/SPON1 Antibody, Clone 3F4 (Cat#: NRZP-0822-ZP4740)
- iNeuMab™ Rabbit Anti-Alpha-synuclein (CBP1631) (Cat#: NAB-08-PZ079)
- iNeuMab™ Mouse Anti-EFNB2 Monoclonal Antibody (CBP1159) (Cat#: NAB-0720-Z4396)
- iNeuMab™ Rabbit Anti-LRRK2 Monoclonal Antibody (CBP1887) (Cat#: NAB-08-PZ735)
- iNeuMab™ Mouse Anti-LRP1 Monoclonal Antibody (CBP3363) (Cat#: NAB-0720-Z6479)
- iNeu™ Human Neural Stem Cell Line (Cat#: NCL200552ZP)
- Mouse Retinal Ganglion Cells (Cat#: NCL2110P145)
- iNeu™ Human Schwann Cell (Cat#: NCL-2103-P63)
- Green Fluorescent Alpha-synuclein Cell Line (Cat#: NCL2110P209)
- Human Brain Astroblastoma U-87 MG (Cat#: NCL2110P117)
- Mouse Retinal Ganglion Cell Line RGC-5 (Cat#: NCL2110P154)
- Rat Immortalized Retinal Muller Cell Line rMC-1 (Cat#: NCL-2106-S93)
- Human Astrocytes (Cat#: NCC20-9PZ01)
- Human Astrocytes, Immortalized (Cat#: NCL-2105-P182-AM)
- iNeu™ Human Motor Neurons (Cat#: NCL-2103-P71)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- Beta Amyloid (1-40), Aggregation Kit (Cat#: NRZP-0323-ZP199)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- VSV-eGFP (Cat#: NTA-2011-ZP20)
- Dextran, NHS Activated (Cat#: NRZP-0722-ZP124)
- AAV2 Full Capsids, Reference Standards (Cat#: NTC2101070CR)
- Tau Antisense Oligonucleotide (IONIS-MAPTRx) (Cat#: NV-2106-P29)
- Lenti of Mouse synuclein, alpha (Snca) transcript variant (NM_001042451) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0864)
- Human presenilin 1 (PSEN1), transcript variant 2 (NM_007318) ORF clone, TurboGFP Tagged (Cat#: NEP-0421-R0140)
- ABCA1 Antisense Oligonucleotide (NV-2106-P27) (Cat#: NV-2106-P27)
- Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2) (NM_020093) ORF clone/lentiviral particle, Myc-DDK Tagged (Cat#: NEP-0621-R0041)
- Human superoxide dismutase 1, soluble (SOD1) (NM_000454) ORF clone, TurboGFP Tagged (Cat#: NEP-0521-R0748)
- Mouse SOD1 shRNA Silencing Adenovirus (Cat#: NV-2106-P14)
- App Rat amyloid beta (A4) precursor protein (App)(NM_019288) ORF clone, Untagged (Cat#: NEP-0421-R0053)
- Lenti of Human TAR DNA binding protein (TARDBP) (NM_007375) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0832)
- Human superoxide dismutase 3, extracellular (SOD3) (NM_003102) ORF clone, Untagged (Cat#: NEP-0521-R0808)
- NeuroBiologics™ Mouse Cerebrospinal Fluid (Cat#: NRZP-0822-ZP497)
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
- NeuroBiologics™ Monkey Cerebrospinal Fluid (Cat#: NRZP-0822-ZP495)
- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroPro™ Anti-NAGLU BBB Shuttle Protein (Cat#: NRZP-0423-ZP506)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein (Cat#: NRZP-0423-ZP510)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein (Cat#: NRZP-0423-ZP501)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein (Cat#: NRZP-0423-ZP500)
- NeuroPro™ Anti-ASA BBB Shuttle Protein (Cat#: NRZP-0423-ZP504)
- NeuroPro™ Anti-IDS BBB Shuttle Protein (Cat#: NRZP-0423-ZP503)
- NeuroPro™ Anti-idursulfase BBB Shuttle Protein (Cat#: NRZP-0423-ZP497)
- NeuroPro™ Anti-PON1 BBB Shuttle Protein (Cat#: NRZP-0423-ZP507)
- NeuroPro™ Anti-SGSH BBB Shuttle Protein (Cat#: NRZP-0423-ZP505)
- NeuroPro™ Anti-Erythropoietin BBB Shuttle Protein (Cat#: NRZP-0423-ZP499)