Mouse Glioma Cell Line GL261

Species:: Mouse

Applications:: Cell Culture

Cell Types:: Tumor Cells

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Product Overview

Neurological Disease Models

Brain Tumor-related Cells

Cell Types

Tumor Cells

Applications

Cell Culture

Relevant Diseases

Glioma

Species

Mouse

Properties

Size

>1x10^6 Cells

Growth Pattern

Adherent

Morphology

fibroblast-like

Culture Medium

DMEM + 10%FBS + 1%GlutaMAX-1 Glutamine + 1% HEPES 1M Buffer solution + 1% P/S

Freeze Medium

90%FBS+10%DMSO

Tissue Source

Brain

Shipping

Dry ice.

Storage

Liquid Nitrogen

Handling Advice

Passage ratio:1:2-1:5; Changing cell medium: 2~3 times/week

Research Use Only

For research use only

Quality Control

• Each vial contains ≥ 1X10^6 viable cells.
• Cells are tested by PCR and are negative for Hepatitis A, B, C, HPV, Herpes and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.

Donor Information

Donor Health

Tumor

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

A solid model

We have been using the GL261 cell line for over a year now. The cells are easy to culture and maintain, with a robust growth pattern that suits our experimental needs perfectly.

Excellent

Handling the GL261 cells is straightforward

They exhibit robust growth both in culture and in animal models. This consistency is vital for our research on glioma therapies.

Excellent

The cells are robust and maintain consistent growth rates

The GL261 cell line is our go-to for glioma research. Its adaptability to various experimental conditions, including hypoxia, makes it a versatile tool in our investigations.

Excellent

We have successfully used GL261 cells to generate genetically modified glioma models

The cell line's compatibility with various genetic manipulation techniques makes it a versatile tool in our laboratory, providing a meaningful model for our research.

Excellent

An excellent tool for our research

Our team has used GL261 cells in both 2D and 3D culture systems. They perform well in both environments, providing versatile options for our research on tumor microenvironments and cell interactions.

Q&As

Could you please tell us the composition of cell cryopreservation solutions and additive?

The cells are cryopreserved in 90%FBS + 10% DMSO.

Are there any specific markers or genetic mutations associated with the mouse glioma cell line GL261?

The GL261 cell line is commonly used as a model for high-grade gliomas and expresses markers such as GFAP, Nestin, and Sox2. It also harbors mutations in the Pten and Trp53 genes, which are commonly found in human glioblastomas.

What is the passage number of the mouse glioma cell line GL261 provided for research projects?

The GL261 cell line is typically provided at a low to mid-passage number to ensure optimal growth and consistency in research experiments. Researchers can expand the cells in culture and maintain their characteristics over multiple passages.

What biosafety level is necessary for handling the GL261 cells, and are there any specific safety precautions I should take?

Handling GL261 cells requires Biosafety Level 2 (BSL-2) containment due to their origin from a cancerous tumor. It is essential to follow standard BSL-2 practices, which include using personal protective equipment (PPE) such as lab coats, gloves, and eye protection, as well as working within a biological safety cabinet to prevent aerosol exposure.