Human Brain Microvascular Endothelial Cells

Species:: Human

Cell Types:: BBB-related Cells

Cell Subtypes:: Brain Endothelial Cells

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Product Overview

Cell Types

BBB-related Cells

Cell Subtypes

Brain Endothelial Cells

Species

Human

Properties

Size

500,000+ Cells frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Frozen (Dry Ice/Liquid Nitrogen)

Storage

Keep frozen in liquid nitrogen until plating

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Donor Information

Donor Health

Normal

Publications

Publications (0)

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Case Studies

Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

We have achieved reproducible results

The cells we ordered have been instrumental in our research on blood-brain barrier integrity. The cells were viable and proliferated well, making our experiments highly successful. We particularly appreciated the comprehensive datasheet that accompanied the product, providing all the necessary culture conditions and handling instructions.

Excellent

HBMECs is crucial for our in vitro models

These cells demonstrated excellent morphology and were responsive to our experimental conditions. The supplier’s customer service was top-notch, addressing our queries promptly and effectively. We observed consistent results across different batches, which speaks to the reliability of the product.

Excellent

We have recommended this product to several colleagues in our department

The cells arrived in excellent condition, and their adherence and growth rates were optimal. The ability to maintain a monolayer for extended periods was particularly beneficial for our permeability assays. The technical support provided was also highly knowledgeable, helping us troubleshoot some initial culture issues.

Excellent

Overall, the quality and reliability of these cells have greatly enhanced our research capabilities

They were healthy and showed high viability, even after several passages. The comprehensive user manual was particularly helpful, providing step-by-step instructions for successful cell culture. We have obtained reproducible and reliable data, which is critical for our grant applications.

Excellent

Using HBMECs from this supplier has greatly facilitated our research

We found the provided culture media to be perfectly suited for maintaining cell health and function. The technical support team was very knowledgeable and responsive, helping us optimize our experimental conditions.

Q&As

What's the appropriate culture medium for Brain Microvascular Endothelial Cells?

Human Endothelial-Growth Media, Cat.# NCL-2103-P178

Can these cells be used for 3D BBB model culture?

Yes, you can purchase these cells together with Human Brain Microvascular Pericytes (HBMVPCs), Cat.#NCL-2103-P229, and Pericyte-Growth Media, Cat.# NCL-2103-P234.

Do you provide ready-to-use 3B BBB models?

We do offer a 3D Human Blood Brain Barrier Model [CAT#: NCL-2103-P187], which can be customized according to your requirements. Please note that the preparation of this model will take approximately 4 weeks.