Human cleaved microtubule-associated protein tau (C-MAPT/C-TAU) ELISA kit

[CAT#: NRZP-0722-ZP222]

For the quantitative determination of human cleaved microtubule-associated protein tau (C-MAPT/C-TAU) concentrations in serum, plasma, cerebrospinal fluid (CSF), tissue homogenates, cell lysates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human C-MAPT/C-TAU.

Species Reactivity

Human

Relevant Diseases

Alzheimer's Disease

Marker

cleaved form of DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU, G protein beta1/gamma2 subunit-interacting factor 1|microtubule-associated protein tau, isoform 4

Properites

Sensitivity

In most cases, the sensitivity is below 15.6 pg/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, cerebrospinal fluid (CSF), tissue homogenates, cell lysates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

62.5 pg/ml-4000 pg/ml

Max Emission

450 nm

Target Details

Target

Tau

Official Name

MAPT

Full Name

microtubule associated protein tau

Alternative Names

DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU, G protein beta1/gamma2 subunit-interacting factor 1, microtubule-associated protein tau, isoform 4

Gene ID

4137(Human)

Uniprot ID

P10636(Human)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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