Human cleaved microtubule-associated protein tau (C-MAPT/C-TAU) ELISA kit
[CAT#: NRZP-0722-ZP222]
For the quantitative determination of human cleaved microtubule-associated protein tau (C-MAPT/C-TAU) concentrations in serum, plasma, cerebrospinal fluid (CSF), tissue homogenates, cell lysates.
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Product Overview
Description
This assay has high sensitivity and excellent specificity for detection of human C-MAPT/C-TAU.
Species Reactivity
Human
Relevant Diseases
Alzheimer's Disease
Marker
cleaved form of DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU, G protein beta1/gamma2 subunit-interacting factor 1|microtubule-associated protein tau, isoform 4
Properites
Sensitivity
In most cases, the sensitivity is below 15.6 pg/ml.
Detection Method
Sandwich
Sample Type
serum, plasma, cerebrospinal fluid (CSF), tissue homogenates, cell lysates
Assay Type
quantitative
Assay Duration
1-5h
Number Of Samples
50-100ul
Detection Range
62.5 pg/ml-4000 pg/ml
Max Emission
450 nm
Target Details
Target
Tau
Official Name
MAPT
Full Name
microtubule associated protein tau
Alternative Names
DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU, G protein beta1/gamma2 subunit-interacting factor 1, microtubule-associated protein tau, isoform 4
Gene ID
4137(Human)
Uniprot ID
P10636(Human)
Protocols
Protocols
1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
Publications
Publications (0)
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Neural Genes
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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