Human Glioblastoma Cells, GFP Expressed

[CAT#: NCL-2103-P207]

Species:: Human

Cell Types:: Tumor Cells

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Product Overview

Description

The centrifugal force method was used to isolate human glioblastoma cells (U87 MG) from brain glioblastoma tissue. GFP-labeled human glioblastoma cells (GFP-U87MG) were selected after infection of U87MG cells with Zeocin RFP-expressing lentivirus. The cells are packed in frozen vials, each vial contains 5 × 10^5 cells. It is recommended to use Universal Whole Growth Medium (NCL-2103-P220) for cell culture. When cultured in accordance with the detailed procedures below, these cells have an average minimum number doubling level>20.

Neurological Disease Models

Brain Tumor-related Cells

Cell Types

Tumor Cells

Relevant Diseases

Glioblastoma

Species

Human

Properties

Size

500,000 Cells

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Liquid Nitrogen (Dry Ice)

Storage

Store the frozen vials of cells in a -80°C freezer for short period storage or in a liquid nitrogen tank for long-term storage.

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Donor Information