Human iPS Cells (inducible Ngn2)

[CAT#: NCL-2103-P81]

Species:: Human

Cell Types:: iPSC

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Product Overview

Description

The human iPS cell line from Creative Biolabs can express Ngn2 when treated with the antibiotic doxycycline (Dox). Inducible Ngn2, which has been successfully incorporated into the human AAVS1 "safe harbor" site, and prefabricated footprint-free human iPS cells are used to create the cell line. It gives easy access to a quick and efficient method of differentiating human iPS cells into excitatory neurons. The incorporation of the "Safe Harbor" website can guarantee the secure and dependable expression of Ngn2 when Dox is made available.

Cell Types

iPSC

Species

Human

Properties

Size

5x10^5 cells/vial

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice - Overnight

Storage

Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

Human iPS cells (inducible Ngn2) were grown in feeder-free conditions with mTeSR1 medium. Each lot of hiPSCs is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and Oct4.

Donor Information