Creative Biolabs

Human iPSC-Derived Glutamatergic Neurons

[CAT#: NCL-2101-ZP42]

Species:
Human
Applications:
Cell Assay
Cell Types:
GlutaNeuron

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Description
Creative Biolabs's iPSC-derived glutamatergic neurons have been reprogrammed using opti-ox (a precise reprogramming technique). Based on this novel technology, human stem cells can be transformed into stable and functionally mature glutamatergic neurons within a few days, providing a high-quality human model for studying neural activity and diseases.

Creative Biolabs's iPSC-derived glutamatergic neuron culture is mainly composed of glutamatergic neurons (>80%) and is characterized by the expression of glutamate transporter genes VGLUT1 and VGLUT2. The remaining minor part of the neuron population expresses cholinergic neuron marker genes. A large number of RNA-seq analysis showed that Glutamatergic neurons have the identity of the nasal central nervous system and express the classic cortical manufacturing genes FOXG1 and TBR1 (data not shown).

Featues:
Karyotype: Normal
Seeding Density: 30,000 cells/cm2
Seeding compatibility: 6-, 12-, 24-, 96- and 384-well compatible
Quality control: ICC and gene expression analysis
Research applications: Academic research, Drug development, Neurotoxicology, Genetic screening (e.g. CRISPR screening).
Cell Types
GlutaNeuron
Applications
Cell Assay
Species
Human
Formulation
Constituent: 10% DMSO
Biosafety Level
1
Cell Viability
>85%
Mycoplasma Testing
Yes
Storage
Shipped on Dry Ice. Store in liquid nitrogen.
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Each batch of cells has passed the high-level expression of Nestin and Sox 2 as well as its self-renewal and multi-lineage differentiation ability. The cells also showed normal karyotypes, and mycoplasma tests were negative by chromosome spread assessment. The blood of pregnant women has been screened for HIV, HTLV, hepatitis B and C.
Gender
Male
For Research Use Only. Not For Clinical Use.
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