Human iPSC-Derived Glutamatergic Neurons
Creative Biolabs's iPSC-derived glutamatergic neuron culture is mainly composed of glutamatergic neurons (>80%) and is characterized by the expression of glutamate transporter genes VGLUT1 and VGLUT2. The remaining minor part of the neuron population expresses cholinergic neuron marker genes. A large number of RNA-seq analysis showed that Glutamatergic neurons have the identity of the nasal central nervous system and express the classic cortical manufacturing genes FOXG1 and TBR1 (data not shown).
Seeding Density: 30,000 cells/cm2
Seeding compatibility: 6-, 12-, 24-, 96- and 384-well compatible
Quality control: ICC and gene expression analysis
Research applications: Academic research, Drug development, Neurotoxicology, Genetic screening (e.g. CRISPR screening).
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.