Creative Biolabs

Human iPSC-Derived Neural Stem Cells - Huntington's Disease Patient

[CAT#: NCL-2101-ZP10]

Can be used with striatal neuron medium kit (Cat. NCC-2101-ZP14) to generate disease-relevant medium spiny neurons.

Species:
Human
Applications:
Cell Assay
Cell Types:
Neural Stem Cell

Datasheet MSDS Request COA

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Bulk Quote
Description
We have applied our innovative technology to develop human neural stem cells directly differentiated from Huntington's Disease iPS cells. Our cells allow Huntington's Disease researchers to build their own human cellular models in a disease-relevant genetic and tissue context.

Our ready-to-use kits allow you to concentrate on your experiments rather than spending valuable time and resources generating Huntington's Disease NSCs in your lab.

HD NSCs are ready made and easy to differentiate. By using our striatal neuron medium, they are able to differentiate to medium spiny neurons expressing key markers, such as DARPP32, CALBINDIN, GABA, CTIP2 and MAP2. By using our cerebral cortical differentiation medium kits, they are capable of differentiating into a spectrum of cortical excitatory and inhibitory neurons and remain viable in culture for months, facilitating reproducible and long-term studies.

Whether you want to study Huntington's Disease pathogenesis at the cellular level, perform neurotoxicity testing or pre-clinical drug screening, we believe that HD NSCs, alongside our optimized culture reagents provide the perfect culture system for your research success.

Advantages of Creative Biolabs HD NSCs
• Human cells derived from diagnosed Huntington's Disease patients
• Available in industrial quantity
• High purity neural stem cell population
• Ready-made and easy to differentiate to either cerebral cortical or striatal neuron lineages
• Robust enough to undergo genetic manipulation
• Capable of differentiating to a spectrum of excitatory and inhibitory neurons
• Remain viable in culture for months to facilitate reproducible and long term studies
Cell Types
Neural Stem Cell
Applications
Cell Assay
Application Notes
• Studying Huntington's Disease pathogenesis at the cellular level
• Studying neural network formation and signal transmission in Huntington's Disease neural culture
• Neurotoxicity testing
• Preclinical drug efficacy testing
• Co-culture studies with astrocytes
Species
Human
Size
1.5 million cells & Neural Plating-XF Medium
Growth Pattern
Adherent
Tissue Source
Fibroblasts (48 Yr Female)
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Each batch of cells has passed the high-level expression of Nestin and Sox 2 as well as its self-renewal and multi-lineage differentiation ability. The cells also showed normal karyotypes, and mycoplasma tests were negative by chromosome spread assessment. The blood of pregnant women has been screened for HIV, HTLV, hepatitis B and C.
Additional Donor Information
CAG repeat expansion 50
For Research Use Only. Not For Clinical Use.
Send Inquiry Send Inquiry
Inquiry Basket
compare

Go to compare