Product
Human M059J Cells
[CAT#: NCB2108FY0053]
Human cells
- Species:
- Human
- Applications:
- 3D Cell Culture
- Cell Types:
- Glia
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Incubate the culture at 37°C in a suitable incubator.
Cell Types
Glia
Applications
3D Cell Culture
Application Notes
Together, M059K and M059J provide a useful model system in which to study the role of DNA protein kinase in cellular and molecular processes involving DNA damage recognition and repair
Relevant Diseases
Malignant Glioblastoma
Research Areas
Neural Cell
Marker
aneuploid; Y chromosome is present
Species
Human
Properties
Form
Frozen
Growth Pattern
Adherent
Morphology
Fibroblast
Culture Medium
These cells are grown in a medium containing a 1:1 mixture of Dulbecco's Modified Eagle's Medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES, 0.5 mM sodium pyruvate, and 1.2 g/L sodium bicarbonate supplemented with 0.05 mM non-essential amino acids and 10% fetal bovine serum.
Freeze Medium
Complete growth medium supplemented with 5% (v/v) DMSO
Tissue Source
Brain
Age
33 years
Shipping
Dry ice
Storage
Vapor phase of liquid nitrogen
Handling Advice
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
Research Use Only
For research use only
Donor Information
Gender
Male
Additional Donor Information
M059J cells were isolated from a tumor specimen from a 33-year-old man with untreated glioblastoma
These cells were simultaneously isolated from the same tumor specimen as M059K (see CRL-2365).
These cells were simultaneously isolated from the same tumor specimen as M059K (see CRL-2365).
Publications
Publications (0)
Neural Cell Culture
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
