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Creative Biolabs
Product

Human M059K Cells

[CAT#: NCB2108FY0054]

Human cells

Species:
Human
Applications:
3D Cell Culture
Cell Types:
Glia

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Product Overview

Description

Incubate the culture at 37°C in a suitable incubator. A 95% Air, 5% CO2 in air atmosphere is recommended if using the medium described on this product.

Cell Types

Glia

Applications

3D Cell Culture

Application Notes

These cell lines provide useful model systems in which to study the role of DNA protein kinase in cellular and molecular processes involving DNA damage recognition and repair.

Relevant Diseases

Malignant Glioblastoma

Research Areas

Neural Cell

Marker

Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%

Species

Human
Properties

Form

Frozen

Growth Pattern

Adherent

Morphology

Fibroblast

Culture Medium

These cells are grown in a medium containing a 1:1 mixture of Dulbecco's Modified Eagle's Medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES, 0.5 mM sodium pyruvate, and 1.2 g/L sodium bicarbonate supplemented with 0.05 mM non-essential amino acids and 10% fetal bovine serum.

Tissue Source

Brain

Age

33 years

Shipping

Dry ice

Storage

Vapor phase of liquid nitrogen

Handling Advice

Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.

Research Use Only

For research use only
Donor Information

Gender

Male

Additional Donor Information

M059K cells were isolated from a tumor specimen from a 33-year-old male patient with untreated glioblastoma. These cells were simultaneously isolated from the same tumor specimen as M059J.
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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