Creative Biolabs

Human Microglia - SV40, Immortalized

[CAT#: NCL-2105-P194-AM]

These cells can be used for disease modeling, drug screening and toxicology testing.

Species:
Human
Applications:
Cell Assay
Cell Types:
Microglia

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Description
Microglia are the resident macrophages of the brain and spinal cord. They are the first and main form of active immune defense in the nervous system. The immortalized human microglia-SV40 cell line is derived from the primary human small Glial cells (>99% purity) and maintain the microglial cell markers CD68 and NGF. These cells are suitable for studying the health and disease of human microglial cells.
Expression profile: NGF, Iba1, TREM2, CD11b, CD68
Cell Types
Microglia
Applications
Cell Assay
Application Notes
The basic medium for this cell line is PriNeu III medium, available from Creative Biolabs, catalog number NCL-2105-P169-AM. To make a complete growth medium, add the following ingredients to the basic medium: The final concentration of bovine serum (NCL -2105-P170-AM)* is 10%, and the final concentration of Pen/Strep solution (NCL-2105-P172-AM) is 1%.
Change the media every 2-3 days.
Carbon dioxide (CO2): 5%, temperature: 37.0°C.
* Unless otherwise specified, do not use heat-inactivated FBS for cell culture.
Species
Human
Freeze Medium
90% FBS+10% DMSO
Tissue Source
Brain
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
Negative
Sterility Testing
The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Shipping
Frozen on dry ice
Storage
Liquid nitrogen immediately upon delivery
Research Use Only
For research use only
Quality Control
Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Gender
Female

For Research Use Only. Not For Clinical Use.
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