Creative Biolabs

Human Neural Stem Cells, Cryopreserved, Fetal

[CAT#: NCL-2103-P06]

NSC morphology, growth behavior when grown in supportive conditions

Species:
Human
Applications:
Cell Assay
Cell Types:
Neural Stem Cell

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Description
Human Neural Stem Cells (HNSC) are self-renewing and are produced during the lifetime of an adult through neurogenesis. These pluripotent adult stem cells produce the main phenotype of the nervous system, differentiated into neurons, astrocytes and less Dendritic cells.
HNSC plays an important role in development, learning, and hippocampal plasticity. They are also used to study age-related decline in proliferation, as well as neurological diseases such as stroke, multiple sclerosis and Parkinson's disease. These cells respond to damage and can differentiate to replace lost or injured neurons. They migrate to brain tumors in a targeted manner and help replace dying neurons in injured adult brain tissue.
Creative Biolabs HNSC is a primary cell derived from the human cortex (a single donor). They were kept frozen in the first generation. After culturing in human neural differentiation medium for 10 days, our HNSC stain is positive for β-tubulin III, GFAP, and the oligodendrocyte marker O4. Each batch was tested for its ability to form neurospheres in the growth medium of human neural stem cells.
Cell Types
Neural Stem Cell
Applications
Cell Assay
Species
Human
Tissue Source
Normal healthy human brain cortex
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
For Research Use Only. Not For Clinical Use.
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