Human phosphorylated microtubule-associated protein tau (pMAPT /pTAU) ELISA kit

[CAT#: NRZP-0722-ZP223]

For the quantitative determination of human phosphorylated microtubule-associated protein tau (pMAPT/pTAU) concentrations in serum, plasma, cerebrospinal fluid (CSF).

Datasheet MSDS Request COA

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human pMAPT.

Species Reactivity

Human

Relevant Diseases

Alzheimer's Disease

Marker

phosphorylated microtubule-associated protein tau (pMAPT /pTAU)

Principle

This Human pMAPT /pTAU ELISA Kit was designed for the quantitative measurement of Human pMAPT /pTAU protein in serum, plasma, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

Properites

Sensitivity

In most cases, the sensitivity is below 7.8 pg/ml.

Detection Method

Sandwich

Sample Type

serum, plasma and cerebrospinal fluid (CSF)

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

31.25 pg/ml-2000 pg/ml

Max Emission

450 nm

Target Details

Target

Tau

Official Name

MAPT

Full Name

microtubule associated protein tau

Alternative Names

DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU, G protein beta1/gamma2 subunit-interacting factor 1, microtubule-associated protein tau, isoform 4

Gene ID

4137(Human)

Uniprot ID

P10636(Human)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

Publications