
Human phosphorylated microtubule-associated protein tau (pMAPT /pTAU) ELISA kit
For the quantitative determination of human phosphorylated microtubule-associated protein tau (pMAPT/pTAU) concentrations in serum, plasma, cerebrospinal fluid (CSF).
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Description
Species Reactivity
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Marker
Principle
Sensitivity
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Sample Type
Assay Type
Assay Duration
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Detection Range
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Target
Official Name
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Protocols
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
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