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Creative Biolabs
Product

Human protein(peptidylprolyl cis/trans isomerase)NIMA-interacting 1, PIN1 ELISA Kit

[CAT#: NRZP-0722-ZP281]

For the quantitative determination of human protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1 (PIN1) concentrations in serum, plasma, urine, tissue homogenates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human PIN1.

Species Reactivity

Human

Marker

Peptidylprolyl cis/trans isomerase, NIMA-interacting 1

Principle

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PIN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Properites

Sensitivity

0.39 pg/ml

Detection Method

Sandwich

Sample Type

serum, plasma, urine, tissue homogenates

Assay Type

quantitative

Assay Time

3-5 working days

Assay Duration

1-5h

Precision

Intra-assay Precision (Precision within an assay): CV%<8%.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%.
Three samples of known concentration were tested in twenty assays to assess.

Number Of Samples

50-100ul

Detection Range

1.56 pg/ml-100 pg/ml

Max Emission

450 nm
Target Details

Target

PIN1

Official Name

PIN1

Full Name

peptidylprolyl cis/trans isomerase, NIMA-interacting 1

Alternative Names

DOD; UBL5

Gene ID

5300(Human)

Uniprot ID

Q13526(Human)
Protocols

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
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For Research Use Only. Not For Clinical Use.
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