Creative Biolabs

Human SNCA Knockout HEK293T Cell Line

[CAT#: NCL2008ZP496]

Human SNCA knockout cell line

Cell Assay
Cell Types:
HEK 293T

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Product Overview


This is a human SNCA gene knockout HEK293T cell line which achieved by using CRISPR/Cas9 gene editing technology.
Recommended control cell line: Human Wild Type HEK293T cell line (ab255449)

Cryopreservation cell culture medium: Cell Freezing Medium-DMSO serum-free medium supplemented with 10% (v/v) DMSO

Cell Types

HEK 293T

Cell Location



Cell Assay

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2

Passage Number


Knockout Validation

Sanger Sequencing, Western Blot (WB)

Relevant Diseases

Neurological Disorders

Research Areas



Parkinson's Disease Signaling




1 x 10e6 cells/vial, 1 mL


Constituents: 8.7% DMSO, 2% Cellulose, methyl ether

Cell State

Live cells are in mid log-phase growth.

Growth Pattern


STR Analysis

Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12

Biosafety Level


Tissue Source


Cell Purity


Cell Viability


Antibiotic Resistance

Puromycin 1.00µg/ml

Mycoplasma Testing


Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.


Dry ice


Shipped on Dry Ice. Store in liquid nitrogen.

Handling Advice

Upon arrival, the sample vial should be stored in liquid nitrogen vapor phase instead of -80°C. Storage at -80°C will cause loss of vitality.

1. Thaw the vial in a 37°C water bath for about 1-2 minutes.
2. Transfer the cell suspension to a 15 mL conical tube containing pre-warmed 5 mL complete medium DMEM + 10% FBS, and rotate 125 x g for about 5 minutes at room temperature.
3. Resuspend the cell pellet with 1 mL of pre-warmed complete medium DMEM + 10% FBS, and distribute it into a 25 cm2 culture flask, which contains 10 mL of pre-warmed complete medium DMEM + 10% FBS.
4. Incubate the culture with 5% CO2 in a 37°C incubator.
5. The recommended subculture ratio is 1:4-1:6. When cells grow at 80-90% confluence and divide, the cells should be passaged.

Research Use Only

For research use only, not for diagnostic or therapeutic use.


Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.

Quality Control

Mycoplasma was tested for sterility, post-freezing viability, and short-term repeat (STR) analysis for cell line identification. Cytochrome oxidase I (COI) was analyzed for cell line species identification.
Target Details


Alpha Synuclein

Official Name


Alternative Names

SNCA; NACP; PARK1; PARK4; PD1; synuclein alpha

Gene ID

6622 (Human); 20617 (Mouse)

Uniprot ID

P37840 (Human); P37840 (Mouse)
For Research Use Only. Not For Clinical Use.
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