Creative Biolabs

IL-1β Protein, Human

[CAT#: NPP-2011-ZP48]

Species:
Human

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Description
Interleukin-1 beta (rhIL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.Recombinant human Interleukin-1 beta (rhIL-1β) produced in E. coli is a single non-glycosylated polypeptide chain containing 153 amino acids. A fully biologically active molecule, rhIL-1β has a molecular mass of 17.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at Creative Biolabs.
Species
Human
Biological Activity
ED50< 10 pg/ml, measured by the dose-dependent stimulation of mouse D10.G4.1 helper T cells, corresponding to a specific activity of 1.0×108 IU/mg.
Expression Host
E. coli
Amino Acids
APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQVVFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTLQLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNWYISTSQAENM PVFLGGTKGG QDITDFTMQF VSS
Molecule Mass
17.3 kDa, observed by reducing SDS-PAGE.
Formulation
Lyophilized after extensive dialysis against PBS.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Endotoxin Level
<0.2 EU/μg, determined by LAL method.
Shipping
The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Storage
Lyophilized recombinant human Interleukin-1 beta (rhIL-1β) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIL-1β should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Handling Advice
Reconstituted in ddH2O or PBS at 100 μg/ml.
Research Use Only
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Target
IL-1β
Neural Proteins & Peptides
Neural Genes
Neural Antibodies
For Research Use Only. Not For Clinical Use.
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