Creative Biolabs

IL-1β Protein, Porcine

[CAT#: NPP-2011-ZP107]

Species:
Porcine

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Description
Interleukin-1 beta (rpIL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. IL-1α and IL-1β are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. While IL-1α and IL-1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1β stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity.Recombinant Porcine IL-1β produced in E. coli is a single non-glycosylated polypeptide chain containing 154 amino acids. A fully biologically active molecule, rpIL-1β has a molecular mass of 17.7 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at Creative Biolabs.
Species
Porcine
Biological Activity
ED50 < 1ng/ml, measured by the dose-dependent stimulation of mouse D10S cells, corresponding to a specific activity of 1.0× 106 IU/mg.
Expression Host
E. coli
Amino Acids
Ala115-Pro267 (Accession #:P26889), expressed with an N-terminal Met
Accession Number
P26889
Molecule Mass
17.7 kDa, observed by reducing SDS-PAGE.
Formulation
Lyophilized after extensive dialysis against PBS.
Purity
> 95% as analyzed by SDS-PAGE.
Endotoxin Level
< 0.2 EU/μg, determined by LAL method.
Shipping
The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Storage
Lyophilized recombinant Porcine IL-1β, remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Porcine IL-1β should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Handling Advice
Reconstituted in ddH2O or PBS at 100 μg/ml.
Research Use Only
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Target
IL-1β
Neural Proteins & Peptides
Neural Genes
Neural Antibodies
For Research Use Only. Not For Clinical Use.
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