Creative Biolabs

IL-1β Protein, RhesusMacaque

[CAT#: NPP-2011-ZP40]

Species:
Rhesus Macaque

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Description
IL-1 beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine. The 17 kDa mature rhesus IL1β shares 96% aa sequence identity with human IL-1 beta.
Species
Rhesus Macaque
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10.G4.1 cells is less than 10 pg/ml, corresponding to a specific activity of> 1.0 × 108 IU/mg.
Expression Host
E. coli
Amino Acids
APVRSLHCTL RDAQLKSLVM SGPYELKALH LQGQDLEQQVVFSMSFVQGE
ESNDKIPVAL GLKAKNLYLS CVLKDDKPTL QLESVDPKNYPKKKMEKRFV
FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTRGGQDITDFTMQF VSS
Molecule Mass
Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Purity
> 98 % by SDS-PAGE and HPLC analyses.
Endotoxin Level
Less than 1 EU/μg of rRhIL-1β as determined by LAL method.
Shipping
The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Storage
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Handling Advice
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Research Use Only
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Target
IL-1β
Neural Proteins & Peptides
Neural Antibodies
Neural Genes
For Research Use Only. Not For Clinical Use.
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