Creative Biolabs

IL-10 Protein, Rat

[CAT#: NPP-2011-ZP63]

Species:
Rat

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Description
Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine produced by a variety of cell lines including T-cells, macrophages and mast cells. IL-10 is classified as a class-2 cytokine, a set of cytokines including IL-19, IL-20, IL-22, IL-24, and IL-26. IL-10 can inhibit the synthesis of pro-inflammatory cytokines such as IFN-gamma, IL-2, IL-3, TNF and GM-CSF. It also stimulates Th2 responses, but suppresses the antigen-presentation capacity of antigen presenting cells.Recombinant rat Interleukin-10 (IL-10)produced in E. coli is a single non-glycosylated polypeptide chain containing 161 amino acids. A fully biologically active molecule, recombinant rat Interleukin-10 (IL-10) has a molecular mass of 18.7kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at Creative Biolabs.
Species
Rat
Biological Activity
ED50 < 0.5μg/ml, measured by a cell proliferation assay using C6 cells, corresponding to a specific activity of> 2.0× 103 units/mg.
Expression Host
E. coli
Amino Acids
MSKGHSIKGD NNCTHFPVSQ THMLRELRAA FSQVKTFFQKKDQLDNIVLT DSLLQDFKGY LGCQALSEMI KFYLVEVMPQAENHGPEIKE HLNSLGEKLK TLWIQLRRCH RFLPCENKSKAVEQVKNDFN KLQDKGVYKA MNEFDIFINC IEAYVTLKMKN
Accession Number
P29456
Molecule Mass
18.7 kDa, observed by reducing SDS-PAGE.
Formulation
Lyophilized after extensive dialysis against PBS.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Endotoxin Level
< 0.2 EU/μg, determined by LAL method.
Shipping
The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Storage
Lyophilized recombinant rat Interleukin-10 (IL-10) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, IL-10 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Handling Advice
Reconstituted in ddH2O at 100 μg/ml.
Research Use Only
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Target
IL-10
For Research Use Only. Not For Clinical Use.
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