Creative Biolabs

IL-6 Protein, Mouse

[CAT#: NPP-2011-ZP43]

Species:
Mouse

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Description
Interleukin 6 is a multi functional protein that plays important roles in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/macrophages, fibroblasts, hepatocytes, keratinocytes, astrocytes, vascular endothelial cells, and various tumor cells. The production of IL-6 is upregulated by numerous signals including mitogenic or antigenic stimulation, LPS, calcium ionophore, IL-1, IL-2, IFN, TNF, PDGF, and viruses. IL-6 expression in monocytes is inhibited by IL-4 and IL-13.
Species
Mouse
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is less than 0.02 ng/ml, corresponding to a specific activity of> 5 × 107 IU/mg.
Expression Host
E. coli
Amino Acids
MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIVEMRKELCNGN
SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKISSGLLEYHSY
LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKIVLPTPISNAL
LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT
Molecule Mass
Approximately 21.7 kDa, a single non-glycosylated polypeptide chain containing 188 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Purity
> 97 % by SDS-PAGE and HPLC analyses.
Endotoxin Level
Less than 1 EU/μg of rMuIL-6 as determined by LAL method.
Shipping
The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Storage
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Handling Advice
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Research Use Only
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Target
IL-6
For Research Use Only. Not For Clinical Use.
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