iNeu™ GABAergic Induced Neuron Kit [Neuron-Astroglia Co-culture]

Description

iNeu neurons from Creative Biolabs are pure and ready-to-use iPSC-derived glutamatergic or GABAergic inducible neurons and astrocytes. This platform comes closest to real human neurobiology observed in primary cultures, providing the ability to efficiently study human neuronal function in vitro. The co-culture kit contains GABAergic, Astroglia and all media required to establish a co-culture model for functional neuronal studies. Contains glutamate and GABAergic neurons, astrocytes and media Cat. No. NRZP- 0722-ZP2), or only glutamatergic neurons, astrocytes, and culture medium (Cat. No. NRZP-0722-ZP4).

This kit is suitable for a variety of biochemical assays. For example, the effect of a compound on neuronal survival, axonal growth or dendritic arborization can be measured by standard assessment of viability or image-based analysis of labeled cells, respectively. When co-cultured with glial cells, effects on synapse formation and composition, transcriptional programs, and electrophysiology can be tested. Neuronal subtypes can be mixed in different ratios to make well-defined co-cultures for different experimental purposes.

Features

- Real human biology
These cells are closer to real human biology than common animal models or other iPSC-based systems. The kit offers better applicability to predict responses to test reagents.
- Fast and even ripening
The kit develops mature synaptic network activity within 3-4 weeks. These kits showed a synchronous burst phenotype similar to rodent primary cultures.
- Reliable, robust and ready-to-use cultures
This differentiated approach yields a highly defined in vitro system with batch-to-batch consistency that provides reproducible results.
- Flexible modular system
Users can control subtype seeding densities and ratios to tailor cultures to specific projects. Custom kit configurations available.

Quality Control

The maturation and neural network formation potential of iPSC-derived induced neurons has been functionally validated on MEA. All cells are tested for chromosomal integrity and pathogens (i.e. Mycoplasma, etc.). Full characterization is included in the Certificate of Analysis.

Shelf Life

This product is stable for at least 6 months from the date of receiving when stored as directed.