iNeu™ Human Keratinocytes

Species:: Human

Cell Types:: Other Cells

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Product Overview

Cell Types

Other Cells

Species

Human

Properties

Shipping

Dry ice

Storage

Store in liquid nitrogen

Research Use Only

For Research Use Only

Publications

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Great cell viability

The keratinocytes showed excellent viability upon thawing. The cells quickly adhered and proliferated in the recommended medium, maintaining a consistent growth rate throughout my experiments. I'm impressed with the reproducibility and performance of these cells.

Excellent

High purity and consistency

I've been using these human keratinocytes for several months, and their purity and consistency remain high across batches. The cells perform reliably in both 2D and 3D culture systems, making them versatile for various applications.

Excellent

Excellent for wound healing studies

The keratinocytes are well-suited for wound healing models. The cells exhibit proper migration and stratification behaviors, which are critical for my experiments. The responsiveness to growth factors is also remarkable.

Excellent

Good morphology and growth rate

These keratinocytes have a stable growth rate and maintain their morphology over several passages. I’ve used them in gene expression studies, and the results have been reliable. The product is worth the investment.

Excellent

Excellent differentiation potential

Culturing and maintaining these keratinocytes is straightforward. They don’t require excessive handling or special conditions, yet they deliver reliable results. The keratinocytes demonstrated strong differentiation capabilities when exposed to specific media, leading to mature epidermal layers in my studies. The product’s performance exceeded my expectations.

Q&As

What is the passage number of iNeu™ human keratinocytes?

Our keratinocytes are provided at an early passage number to ensure high viability and genetic stability. Lower passage numbers are crucial for maintaining the cells' original characteristics and minimizing the risk of genetic drift, which can affect experimental outcomes. We recommend using these cells within a specified passage range for optimal results.

Are there any recommended controls when using iNeu™ human keratinocytes in experiments?

Yes, when using keratinocytes in your experiments, it is recommended to include appropriate controls such as untreated cells or cells treated with a known agent that induces a specific response. Including controls helps validate the experimental conditions and ensures the reliability of the results. Additionally, depending on the experiment, other cell types like fibroblasts or melanocytes may serve as comparative controls to study specific interactions or effects.

What is the expected lifespan of keratinocytes in culture, and how can I maintain their viability over time?

The lifespan of keratinocytes in culture depends on factors such as passage number, culture conditions, and handling practices. Under optimal conditions, these cells can be cultured for several passages while maintaining their viability and functionality. To extend their lifespan, it is important to monitor the culture closely, avoid over-confluency, and use fresh medium regularly. Ensuring that the cells are not subjected to stressors like excessive passage or suboptimal conditions will help maintain their integrity for your research needs.