iNeu™ Human Keratinocytes

Species:: Human

Cell Types:: Other Cells

Cell Subtypes:: Keratinocytes

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Product Overview

Description

iNeu™ human keratinocytes are derived from human dermal fibroblasts, using a feeder-free, serum-free, genetic-free, virus-free induced pluripotent stem cell (iPSC) line, and have fully defined proprietary induction and maturation conditions. iNeu™ cells can be cultured in a monolayer and can express different biomarkers, such as Cytokeratin-18. iNeu™ can be used under mature conditions, co-culture conditions (used with fibroblasts, melanocytes or other epidermal subtypes), and short-term functional characterization studies.

Cell Types

Other Cells

Cell Subtypes

Keratinocytes

Application Notes

Applications:

Skin disease modeling
Toxicity testing
Phenotypic assays (e.g., expression of differentiation markers)
Mitochondrial dysfunction
Skin models (2D or 3D)
Bioprinting of skin
Studies related to epidermal functions

Species

Human

Properties

Size

~0.6x10^6 cells per 1ml of freezing medium (vial)

Growth Pattern

adherent

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

Sterility, Safety, (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative; Cell viability post-thawing (>90%)

Donor Information

Donor Health

Normal

Publications

Publications (0)

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Scientific Resources

[Brochure]

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Great cell viability

The keratinocytes showed excellent viability upon thawing. The cells quickly adhered and proliferated in the recommended medium, maintaining a consistent growth rate throughout my experiments. I'm impressed with the reproducibility and performance of these cells.

Excellent

High purity and consistency

I've been using these human keratinocytes for several months, and their purity and consistency remain high across batches. The cells perform reliably in both 2D and 3D culture systems, making them versatile for various applications.

Excellent

Excellent for wound healing studies

The keratinocytes are well-suited for wound healing models. The cells exhibit proper migration and stratification behaviors, which are critical for my experiments. The responsiveness to growth factors is also remarkable.

Excellent

Good morphology and growth rate

These keratinocytes have a stable growth rate and maintain their morphology over several passages. I’ve used them in gene expression studies, and the results have been reliable. The product is worth the investment.

Excellent

Excellent differentiation potential

Culturing and maintaining these keratinocytes is straightforward. They don’t require excessive handling or special conditions, yet they deliver reliable results. The keratinocytes demonstrated strong differentiation capabilities when exposed to specific media, leading to mature epidermal layers in my studies. The product’s performance exceeded my expectations.

Q&As

What is the passage number of iNeu™ human keratinocytes?

Our keratinocytes are provided at an early passage number to ensure high viability and genetic stability. Lower passage numbers are crucial for maintaining the cells' original characteristics and minimizing the risk of genetic drift, which can affect experimental outcomes. We recommend using these cells within a specified passage range for optimal results.

Are there any recommended controls when using iNeu™ human keratinocytes in experiments?

Yes, when using keratinocytes in your experiments, it is recommended to include appropriate controls such as untreated cells or cells treated with a known agent that induces a specific response. Including controls helps validate the experimental conditions and ensures the reliability of the results. Additionally, depending on the experiment, other cell types like fibroblasts or melanocytes may serve as comparative controls to study specific interactions or effects.

What is the expected lifespan of keratinocytes in culture, and how can I maintain their viability over time?

The lifespan of keratinocytes in culture depends on factors such as passage number, culture conditions, and handling practices. Under optimal conditions, these cells can be cultured for several passages while maintaining their viability and functionality. To extend their lifespan, it is important to monitor the culture closely, avoid over-confluency, and use fresh medium regularly. Ensuring that the cells are not subjected to stressors like excessive passage or suboptimal conditions will help maintain their integrity for your research needs.