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Creative Biolabs

iNeu™ Human Neural Progenitor Cells

[CAT#: NCL-2103-P80] Q&As(3)

Species:
Human
Cell Types:
Neural Progenitor Cells

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Product Overview

Description

iNeu™ is a neural progenitor cell derived from human iPSC. They are derived from a non-integrated induced pluripotent stem cell (iPSC) line under fully defined proprietary neural induction conditions. iNeu™ cells are polarized when plated in culture as a monolayer and express markers such as Vimentin. We have verified that iNeu™ cells are pluripotent neural and glial progenitor cells, because they can be further differentiated into neural and glial cell types under specific in vitro serum-free/non-integrated reprogramming conditions.

Cell Types

Neural Progenitor Cells

Application Notes

Applications:

Disease modeling
Neurotoxicity testing
Phenotypic Assays (e.g., induction of lineage-specific marker proteins)
Research and development of cell therapies in animal models
Studying neurogenesis, neuronal differentiation and function, and neural network formation

Species

Human
Properties

Growth Pattern

adherent

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

Sterility, Safety, (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative; Cell viability post-thawing (>90%)
Donor Information

Donor Health

Normal
Publications

Publications (0)

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Customer Reviews and Q&As
Q&As
What's the recommend media?
We would recommend the medium NeuCULT™ iNPC Culture Medium [CAT#: NPC-M01]
What is the maximum passage number recommended for these cells?
Cells were banked at passage 3. Upon thawing, the cells are at passage 4. Cells may be expanded for further 3-5 passages.
Do you alao provide the recommended coating solution?
Yes, Laminin, Cat.#NCC200638ZP.
For Research Use Only. Not For Clinical Use.
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