iNeu™ Human Retinal Pigment Epithelials

Species:: Human

Cell Types:: Other Cells

Cell Subtypes:: Retinal Pigment Epithelial Cells

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Product Overview

Description

Retinal pigment epithelium (RPE) is the layer of pigment cells that surrounds the neurosensory retina. These cells support the retinal visual cells, which attach to the choroid below and the visual cells of the upper layer of the retina. RPE has multiple functions: light absorption, epithelial transport, spatial ion buffer, visual circulation, phagocytosis, secretion and immune regulation. iNeu™ are retinal pigment epithelial cells derived from human iPSC. They express biomarkers such as RPE-65, RLBP1 and BEST.

Cell Types

Other Cells

Cell Subtypes

Retinal Pigment Epithelial Cells

Application Notes

Applications:

Disease modeling
Toxicity testing
Phenotypic Screening (e.g., cytokine secretion, expression of differentiation markers)
In vitro assays using RPE cells (e.g., phagocytosis)

Species

Human

Properties

Size

~0.6x10^6 cells per 1ml of freezing medium (vial)

Growth Pattern

adherent

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

Sterility, Safety, (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative; Cell viability post-thawing (>90%)

Donor Information

Donor Health

Normal

Publications

Publications (0)

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Scientific Resources

[Brochure]

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Be invaluable for our studies on retinal diseases

The cells exhibit excellent morphological and functional characteristics, matching the in vivo conditions very closely. Their high purity and consistent performance across multiple batches have significantly advanced our research on retinal degeneration models.

Excellent

The results have been consistently impressive

The cells are well-characterized and maintain their functionality over extended culture periods.

Excellent

A high-quality product

The cells are robust and reliable, providing reproducible results in our experiments. They have been instrumental in our research on retinal cell signaling pathways and drug testing.

Excellent

Highly recommended for retinal research

We've had a great experience with the cell. The cells exhibit strong pigmentation and functional characteristics similar to native retinal pigment epithelium. This has enabled us to explore various aspects of retinal cell biology with high confidence.

Excellent

The retinal pigment epithelials have been an excellent addition to our retinal disease studies

The cells are consistently high quality, and their performance has facilitated our research into retinal cell replacement therapies. The detailed characterization provided is very useful for experimental design.

Q&As

Can they undergo cryopreservation again?

After the initial thawing, the viability of the cells is greater than 90%. We do not advise cryopreservation again.

What culture medium do they require?

We provide Human RPE medium, Cat.#NCL-2103-P70C

What is the doubling time of the cells?

The iRPE passage is typically conducted biweekly, once the cells have developed their polygonal shape and exhibit a slight pigmentation. This pigmentation is usually discernible solely in the cell pellet during the passage process.

How many times they can be subcultured before they stop expressing characteristic features of human RPE?

The iRPE cells can be passaged 3-4 times.