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Creative Biolabs

HOT iNeu™ Human Sensory Neurons

[CAT#: NCL-2103-P62] Review(5) Q&As(3)

Species:
Human
Cell Types:
Sensory Neurons

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Product Overview

Description

iNeu™ cells are human sensory neurons derived from human dermal cells using a human induced pluripotent stem cell (iPSC) line under fully defined proprietary induction and maturation conditions. iNeu's sensory neurons are plated in a monolayer during culture, are branched, and express biomarkers such as β-Tuj (neuron-specific), BRN3a and peripheral proteins. In addition, iNeu™ cells express biomarkers such as ASIC, CNR1, NTRK, P2X4, SCN9a/Nav1.7, TRPA1, TRPV1, P2X3, and SCN10A/Nav1.8 (RT/qPCR data not shown). The sensory neurons can perform various functional characterization and drug candidate validation studies under mature conditions or co-culture conditions (used with Schwann cells or other neuronal subtypes).

Cell Types

Sensory Neurons

Application Notes

Applications:

Disease modeling
Toxicity evaluations
Phenotypic Assays (e.g., expression of differentiation markers)
Research and Development of cell therapies in animal models
Studying neuronal differentiation, function, and myelination
Molecular biomarker studies
Co-Culture Studies (e.g., sensory neurons and schwann cells)

Species

Human
Properties

Size

~0.6x10^6 cells per 1ml of freezing medium (vial)

Growth Pattern

adherent

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

Sterility, Safety, (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative; Cell viability post-thawing (>90%)
Donor Information

Donor Health

Normal
Publications

Publications (0)

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Customer Reviews and Q&As
Customer Reviews Average Customer Ratings Overall
5.0
user
Excellent
The cells were well-preserved and arrived in optimal condition
The sensory neurons arrived on time and were packaged securely. They integrated well into our existing culture systems and have been robust in our electrophysiological studies. We've made significant progress in our research thanks to these high-quality cells.
user
Excellent
A top-quality product from a reliable supplier
We needed high-quality sensory neurons for our drug testing protocols, and these fit the bill perfectly. They were easy to handle and responded well to various treatments. The reproducibility of our results has significantly improved since we started using these neurons.
user
Excellent
Provided us with invaluable insights
The sensory neurons arrived in perfect condition, well-packaged, and with clear instructions. We have successfully used them in multiple projects, studying sensory pathways and disease models. Their performance has been consistent, and we've had great success in differentiating them as needed for our experiments.
user
Excellent
The quality and consistency of the neurons have been excellent
The sensory neurons we received were of exceptional quality. They responded well to both chemical and physical stimuli, providing us with reliable data for our neurochemical analyses. The detailed handling instructions were particularly useful for our team.
user
Excellent
The cells were easy to culture and maintain, and their sensory responses were very robust
The neurons provided by this company are exceptional. The viability of the cells upon arrival was impressive, with over 90% survival rate. The neurons exhibited robust responses to sensory stimuli, and their electrophysiological properties were consistent with what we expect from primary sensory neurons.
Q&As
Can you customize the human sensory neurons to meet specific research needs?
Yes, we can provide customized human sensory neuron cultures tailored to your specific research requirements, such as specific genetic mutations or disease models.
What is the recommended storage and handling procedure for the sensory neurons upon receipt?
Upon receipt, it is recommended to immediately transfer the sensory neurons to a suitable storage environment, typically a cryogenic freezer or liquid nitrogen tank, to maintain long-term viability. Detailed storage and handling instructions are provided with the product to ensure optimal preservation of cell quality and functionality.
Do you provide technical support and troubleshooting assistance?
Yes, comprehensive technical support is included with all purchases. Our team of experts is available to provide guidance on culture protocols, troubleshooting assistance, and experimental design. We are committed to ensuring researchers have the support they need for successful outcomes.
For Research Use Only. Not For Clinical Use.
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