iNeuMab™ Anti-CXCL13 Antibody (NRP-0422-P2082)

Q: How stable is this antibody over time? Can it be stored long-term?

The antibody is highly stable when stored properly. We recommend storing it at -20°C for long-term storage. For short-term use, storing it at 4°C is acceptable. The antibody is stable for up to one year when stored at -20°C. Repeated freeze-thaw cycles should be avoided to maintain its integrity and effectiveness.

Q: What should I do if I observe high background staining in my experiments?

If you encounter high background staining, we recommend optimizing the antibody concentration, as well as the blocking and washing steps. Reducing the antibody concentration or increasing the blocking time may help. Additionally, using a more stringent washing buffer or increasing the number of washing steps can reduce non-specific binding. We also suggest including a no-primary antibody control to identify the source of the background.

Q: Can this antibody be used in combination with fluorescent secondary antibodies?

Absolutely. The antibody can be paired with fluorescently labeled secondary antibodies for immunofluorescence applications. This combination provides bright and specific signals, allowing for detailed visualization of CXCL13 expression in your samples. We recommend selecting a secondary antibody with a fluorophore that suits your microscope’s filter set and considering the use of anti-fade mounting media to preserve fluorescence.

Product Overview

Description

The antibody demonstrated multi-species CXCL13 specificity as bound to recombinant human, mouse and cynomolgus monkey CXCL13.

Species Reactivity

Human; Mouse; Cynomolgus Monkey

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG

Applications

WB; ELISA; IHC; Inhib; Neut; In Vitro; In Vivo

Relevant Diseases

Multiple Sclerosis; Neuroinflammation

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

CXCL13

Official Name

CXCL13

Full Name

chemokine (C-X-C motif) ligand 13

Alternative Names

BCA-1; Angie; C-X-C motif chemokine 13; CXC chemokine BLC; ANGIE2; B lymphocyte chemoattractant; B cell-attracting chemokine 1; BLR1L; Small-inducible cytokine B13; SCYB13; ANGIE; BLC; BCA1

Gene ID

10563(Human);55985(Mouse)

Uniprot ID

O43927(Human);O55038(Mouse)

Publications

Publications (0)

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Scientific Resources

[Brochure]

Neuros Antibody Product Panel

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Excellent specificity and consistency

The antibody has provided excellent specificity in our immunohistochemistry experiments. We’ve used it across several batches, and the results are always consistent. The staining is clear, and the background noise is minimal, making it a reliable tool for our research.

Excellent

Reliable and reproducible results

I've been using the antibody for a few months now, and it consistently delivers reliable results. The antibody binds specifically to CXCL13, with no cross-reactivity observed in our Western blot experiments. The reproducibility of the results is impressive, which is crucial for long-term studies.

Excellent

Great for immunofluorescence applications

This antibody has been a game-changer for our immunofluorescence studies. The signal is strong, and the specificity is top-notch. We've tested it on various tissue samples, and it has worked beautifully every time. The images we obtained were clear and detailed.

Excellent

Strong signal with minimal background

The antibody from NeuroMab has consistently provided strong signals in our ELISA assays with minimal background noise. This has allowed us to obtain more accurate and interpretable data. The antibody’s performance has made it an essential part of our lab’s toolkit.

Excellent

Highly specific and sensitive

In our recent experiments, we needed an antibody with high specificity and sensitivity to detect low levels of CXCL13. The antibody exceeded our expectations, providing clear, distinct signals even at lower concentrations.

Q&As

How stable is this antibody over time? Can it be stored long-term?

The antibody is highly stable when stored properly. We recommend storing it at -20°C for long-term storage. For short-term use, storing it at 4°C is acceptable. The antibody is stable for up to one year when stored at -20°C. Repeated freeze-thaw cycles should be avoided to maintain its integrity and effectiveness.

What should I do if I observe high background staining in my experiments?

If you encounter high background staining, we recommend optimizing the antibody concentration, as well as the blocking and washing steps. Reducing the antibody concentration or increasing the blocking time may help. Additionally, using a more stringent washing buffer or increasing the number of washing steps can reduce non-specific binding. We also suggest including a no-primary antibody control to identify the source of the background.

Can this antibody be used in combination with fluorescent secondary antibodies?

Absolutely. The antibody can be paired with fluorescently labeled secondary antibodies for immunofluorescence applications. This combination provides bright and specific signals, allowing for detailed visualization of CXCL13 expression in your samples. We recommend selecting a secondary antibody with a fluorophore that suits your microscope’s filter set and considering the use of anti-fade mounting media to preserve fluorescence.