iNeuMab™ Anti-SEZ6 Antibody (NRP-0422-P517)

Q: Can this antibody be used for immunohistochemistry on paraffin-embedded tissues?

Yes, the antibody is suitable for immunohistochemistry (IHC) on paraffin-embedded tissues. It has been optimized to work well with both frozen and formalin-fixed, paraffin-embedded (FFPE) tissue sections. The antibody provides strong, specific staining with minimal background, allowing for clear visualization of PVRIG in various tissue types.

Q: What controls should I use when working with the antibody?

When working with the antibody, we recommend including both positive and negative controls in your experiments. For western blotting, use a lysate from a cell line known to express PVRIG as a positive control. For immunohistochemistry or flow cytometry, tissues or cells with confirmed PVRIG expression serve as good controls. Additionally, using a secondary antibody control can help identify any non-specific binding.

Q: What type of secondary antibody is recommended for use with this product?

For optimal results, we recommend using a secondary antibody that is specific to the host species of the antibody, which is typically a mouse monoclonal antibody. A high-quality anti-mouse IgG secondary antibody, conjugated with a detection label such as HRP for western blotting or a fluorophore for immunofluorescence, will provide the best results. The choice of detection label depends on your specific application.

Product Overview

Description

The antibody specifically binds to a human SEZ6 protein, use for the treatment, diagnosis or prophylaxis of neoplastic disorders.

Species Reactivity

Human; Mouse; Rat; Cynomolgus Monkey

Clonality

Monoclonal

Host Species

Mouse

Applications

FC; IHC; In Vitro; In Vivo

Relevant Diseases

Epilepsy

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

SEZ6

Official Name

SEZ6

Full Name

Seizure Related 6 Homolog

Alternative Names

Seizure Related 6 Homolog; Seizure Related 6 Homolog (Mouse); Seizure Protein 6 Homolog; HSEZ-6; BSRPC; SEZ-6;

Gene ID

20370(Mouse); 192247(Rat)

Uniprot ID

Q7TSK2(Mouse)

Publications

Publications (0)

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Scientific Resources

[Brochure]

Neuros Antibody Product Panel

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Great performance in flow cytometry

I've been using the antibody for flow cytometry, and it consistently delivers reliable results. The specificity is top-notch, and the signal is strong with minimal background noise. This antibody has become a staple in my lab for immune checkpoint studies.

Excellent

Highly specific antibody

I’ve tested several antibodies for PVRIG detection, and this one from NeuroMab is by far the best. It binds specifically to the target protein with excellent sensitivity

Excellent

Good signal with minimal background

In my immunohistochemistry experiments, this antibody provided a good signal with minimal background staining. The clarity and precision in detecting PVRIG in tissue sections were impressive. I’m very satisfied with its performance.

Excellent

Perfect for fow cytometry applications

I have used this antibody in several flow cytometry experiments, and it has consistently performed well. The binding affinity is excellent, and the results are reproducible.

Excellent

Exceptional quality and sensitivit

The quality and sensitivity of the antibody are exceptional. It has significantly improved the accuracy of my experiments, particularly in detecting low levels of PVRIG in complex samples.

Q&As

Can this antibody be used for immunohistochemistry on paraffin-embedded tissues?

Yes, the antibody is suitable for immunohistochemistry (IHC) on paraffin-embedded tissues. It has been optimized to work well with both frozen and formalin-fixed, paraffin-embedded (FFPE) tissue sections. The antibody provides strong, specific staining with minimal background, allowing for clear visualization of PVRIG in various tissue types.

What controls should I use when working with the antibody?

When working with the antibody, we recommend including both positive and negative controls in your experiments. For western blotting, use a lysate from a cell line known to express PVRIG as a positive control. For immunohistochemistry or flow cytometry, tissues or cells with confirmed PVRIG expression serve as good controls. Additionally, using a secondary antibody control can help identify any non-specific binding.

What type of secondary antibody is recommended for use with this product?

For optimal results, we recommend using a secondary antibody that is specific to the host species of the antibody, which is typically a mouse monoclonal antibody. A high-quality anti-mouse IgG secondary antibody, conjugated with a detection label such as HRP for western blotting or a fluorophore for immunofluorescence, will provide the best results. The choice of detection label depends on your specific application.