Creative Biolabs

iNeuMito™ Mitochondrial Biosensor Assay using Human Neural Progenitors

[CAT#: NCL-2103-P58]

Species:
Human
Applications:
Cell Assay
Cell Types:
Neural Progenitor Cell

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Description
iNeuMito™ cells contain mitochondrial biosensors integrated into human iPS-derived neural progenitor cells. Mitochondrial dysfunction is related to human nervous system diseases and mental diseases, and neural progenitor cells have been used as cell models. iNeu's biosensor can respond to changes in mitochondrial calcium and report an increase or decrease in calcium content in the 605nm-625nm fluorescence range (excitation/emission). These sensors have an excellent signal-to-noise ratio and can operate in the range of seconds to minutes. This tool provides an effective method for detecting changes in mitochondrial organelles and enables high-throughput screening methods for drug discovery and toxicology screening.
Cell Types
Neural Progenitor Cell
Applications
Cell Assay
Application Notes
Applications:

Neurotoxicity testing for reporting cellular calcium fluctuations
Phenotypic Assays (i.e., mitochondrial toxicity and function)
Chemical compound or small molecules testing
Research and development on animal models for human diseases
Studying neurogenesis, neuronal differentiation and function, and calcium-mediated signal transduction pathways.
Species
Human
Size
~0.6x10^6 cells per 1ml of freezing medium (vial)
Growth Pattern
adherent
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Sterility, Safety, (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative; Cell viability post-thawing (>90%)
For Research Use Only. Not For Clinical Use.
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