Creative Biolabs

iNeuVol™ Cationic Voltage Biosensor Assay using Human Neural Progenitor Cells

[CAT#: NCL-2103-P55]

Species:
Human
Applications:
Cell Assay
Cell Types:
Neural Progenitor Cell

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Description
The cation voltage biosensor iNeuVol™ responds as a cellular fluorescent reporter (excitation/emission: 610nm / 625nm) and is integrated into iNeu's iPSC-derived neural progenitor cell iNStem. iNStem is derived from a non-integrated induced pluripotent stem cell (iPSC) line under fully defined proprietary neural induction conditions. When cultured in a monolayer, iNeuCal™-iNStem cells have a polarized structure and express markers such as Vimentin. We have verified that iNStem™ cells are pluripotent neural and glial progenitor cells because they can be further differentiated into neural and glial cell types under specific in vitro reprogramming conditions. The iNeuVol™ biosensor is introduced into cells using a proprietary liposome-mediated method.
Cell Types
Neural Progenitor Cell
Applications
Cell Assay
Application Notes
Applications:

Chemical compound or small molecules testing
Phenotypic Assays
Neurotoxicity testing for reporting cationic flux
Signal transduction pathway modulations
Ion Channel studies
Chemical compound or small molecules testing or screening
For low, medium, or high-throughput applications
Species
Human
Size
~0.6x10^6 cells per 1ml of freezing medium (vial)
Growth Pattern
adherent
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Sterility, Safety, (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative; Cell viability post-thawing (>90%)
For Research Use Only. Not For Clinical Use.
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