Lenti of Mouse TAR DNA binding protein (Tardbp) transcript variant 1 (NM_145556) ORF clone, mGFP Tagged

Description

This is a mouse TDP43 mGFP tagged ORF clone expression plasmid, ready for transfecting into mammalian cells.

Gene/Insert Name

TDP43

Species

Mouse

Insert Size (bp)

1245 bp

Tag

mGFP

Type

Expression Vector/ Viral Particle

Vector

pLenti

E. coli Selection

Chloramphenicol (34 ug/mL)

Cell Selection

Puromycin

ORF Nucleotide Sequence

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGTCTGAATATATTCGGGTAACAGAAGATGAGAACGATGAACCCATTGAAATACCATCAGAAGACGATGGGACGGTGTTGCTGTCCACAGTTACAGCCCAGTTTCCAGGGGCATGCGGCCTGCGCTACCGGAATCCCGTGTCTCAGTGTATGAGAGGAGTCCGACTGGTGGAAGGAATTCTGCATGCCCCAGATGCTGGCTGGGGCAATCTGGTATATGTTGTCAACTATCCCAAAGATAACAAAAGGAAAATGGATGAGACAGATGCTTCCTCTGCAGTGAAAGTGAAAAGAGCAGTCCAGAAAACATCTGACCTCATAGTGTTGGGTCTCCCCTGGAAAACAACTGAGCAGGATCTGAAAGACTATTTCAGTACTTTTGGAGAGGTTCTTATGGTTCAGGTCAAGAAAGATCTTAAAACTGGTCACTCGAAAGGGTTTGGCTTTGTTCGATTTACAGAATATGAAACCCAAGTGAAAGTAATGTCACAACGACATATGATAGATGGGCGATGGTGTGACTGTAAACTTCCCAACTCTAAGCAAAGCCCAGACGAGCCTTTGAGAAGCAGAAAGGTGTTTGTTGGACGTTGTACAGAGGACATGACTGCTGAAGAGCTTCAGCAGTTTTTCTGTCAGTATGGAGAAGTGGTAGATGTCTTCATTCCCAAACCATTCAGAGCTTTTGCCTTCGTCACCTTTGCAGATGATAAGGTTGCCCAGTCTCTTTGTGGAGAGGATTTGATCATTAAAGGAATCAGCGTGCATATATCCAATGCTGAACCTAAGCATAATAGCAATAGACAGTTAGAAAGAAGTGGAAGATTTGGTGGTAATCCAGGTGGCTTTGGGAATCAGGGTGGGTTTGGTAACAGTAGAGGGGGTGGAGCTGGCTTGGGAAATAACCAGGGTGGTAATATGGGTGGAGGGATGAACTTTGGTGCTTTTAGCATTAACCCAGCGATGATGGCTGCGGCTCAGGCAGCGTTGCAGAGCAGTTGGGGTATGATGGGCATGTTAGCCAGCCAGCAGAACCAGTCGGGCCCATCTGGGAATAACCAAAGCCAGGGCAGCATGCAGAGGGAACCAAATCAGGCTTTTGGTTCTGGAAATAATTCCTACAGTGGTTCTAATTCTGGTGCCCCCCTTGGTTGGGGGTCAGCATCAAATGCAGGATCGGGCAGTGGTTTTAATGGGGGCTTTGGCTCGAGCATGGATTCTAAGTCTTCTGGCTGGGGAATGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA

Protein Sequence

MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKDYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSPDEPLRSRKVFVGRCTEDMTAEELQQFFCQYGEVVDVFIPKPFRAFAFVTFADDKVAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGGNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQSQGSMQREPNQAFGSGNNSYSGSNSGAPLGWGSASNAGSGSGFNGGFGSSMDSKSSGWGMTRTRPLEQKLISEEDLAANDILDYKDDDDKV

Restriction Sites

SgfI-MluI

Application Notes

The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.

Purification

Ion-exchange column purified

Research Areas

Neural Signal Transduction

Relevant Diseases

Amyotrophic Lateral Sclerosis

Key Components

Transfection-ready dried plasmid DNA

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at-20°C. The DNA is stable for at least one year from date of shipping when stored at-20°C

Research Use Only

For research use only

Target

TDP43

Official Name

TDP43

Full Name

TAR DNA-binding protein 43

Alternative Names

TARDBP; ALS10; TDP-43; TAR DNA binding protein

Gene ID

23435(Human); 230908(Mouse); 298648(Rat)

Uniprot ID

Q13148(Human); Q921F2(Mouse)