Lenti of Rat clusterin (Clu) (NM_053021) ORF clone, Myc-DDK Tagged

Description

This is a rat CLU Myc-DDK tagged ORF clone expression plasmid, ready for transfecting into mammalian cells.

Gene/Insert Name

CLU

Species

Rat

Insert Size (bp)

1341 bp

Tag

Myc-DDK

Type

Expression Vector/ Viral Particle

Vector

pLenti

E. coli Selection

Chloramphenicol (34 ug/mL)

Cell Selection

Puromycin

ORF Nucleotide Sequence

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGAAGATTCTCCTGCTGTGTGTGGCACTGCTGCTGACCTGGGACAATGGCATGGTCCTGGGAGAGCAGGAGTTCTCTGACAATGAGCTCCAAGAACTGTCCACTCAAGGAAGTAGGTATGTTAATAAGGAGATTCAGAACGCCGTCCAGGGGGTGAAGCACATAAAGACCCTCATAGAAAAAACCAACGCAGAGCGCAAGTCCCTGCTCAACAGTTTAGAGGAAGCCAAAAAGAAGAAAGAGGGTGCTCTAGATGACACCAGGGATTCTGAAATGAAGCTGAAGGCTTTCCCGGAAGTGTGTAACGAGACCATGATGGCCCTCTGGGAAGAGTGTAAGCCCTGCCTGAAGCACACCTGCATGAAGTTCTACGCACGCGTCTGCAGGAGCGGCTCGGGGCTGGTTGGTCGCCAGCTAGAGGAGTTTCTGAACCAGAGCTCACCCTTCTACTTCTGGATGAACGGGGACCGCATCGACTCCCTGCTGGAGAGTGACCGGCAGCAGAGCCAAGTCCTAGATGCTATGCAGGACAGCTTCACTCGGGCGTCTGGCATCATACATACGCTTTTCCAGGACCGGTTCTTCACCCATGAGCCCCAGGACATCCACCATTTCTCCCCCATGGGCTTCCCACACAAGCGGCCTCATTTCTTGTACCCCAAGTCCCGCTTGGTCCGCAGCCTCATGCCTCTCTCCCACTACGGGCCTCTGAGCTTCCACAACATGTTCCAGCCTTTCTTTGATATGATACACCAGGCTCAACAGGCCATGGACGTCCAGCTCCATAGCCCAGCTTTACAGTTCCCGGATGTGGATTTCTTAAAAGAAGGTGAAGATGACCCGACAGTGTGCAAGGAGATCCGCCATAACTCCACAGGATGCCTGAAGATGAAGGGCCAGTGTGAGAAGTGCCAAGAGATCTTGTCTGTGGACTGTTCGACCAACAATCCTGCCCAGGCTAACCTGCGCCAGGAGCTAAACGACTCGCTCCAGGTGGCTGAGAGGCTGACCCAGCAGTACAACGAGCTGCTTCATTCCCTCCAGTCCAAGATGCTCAACACCTCATCCCTGCTGGAACAGCTGAACGACCAGTTCACGTGGGTGTCCCAGCTGGCTAACCTCACACAGGGCGATGACCAGTACCTTCGGGTCTCCACAGTGACAACCCATTCTTCTGACTCAGAAGTCCCCTCTCGTGTCACTGAGGTGGTGGTGAAGCTGTTTGACTCTGACCCCATCACAGTGGTGTTACCAGAAGAAGTCTCCAAGGATAACCCTAAGTTTATGGACACAGTGGCAGAGAAAGCGCTACAGGAATACCGCAGGAAAAGCCGCATGGAAAGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA

Protein Sequence

MKILLLCVALLLTWDNGMVLGEQEFSDNELQELSTQGSRYVNKEIQNAVQGVKHIKTLIEKTNAERKSLLNSLEEAKKKKEGALDDTRDSEMKLKAFPEVCNETMMALWEECKPCLKHTCMKFYARVCRSGSGLVGRQLEEFLNQSSPFYFWMNGDRIDSLLESDRQQSQVLDAMQDSFTRASGIIHTLFQDRFFTHEPQDIHHFSPMGFPHKRPHFLYPKSRLVRSLMPLSHYGPLSFHNMFQPFFDMIHQAQQAMDVQLHSPALQFPDVDFLKEGEDDPTVCKEIRHNSTGCLKMKGQCEKCQEILSVDCSTNNPAQANLRQELNDSLQVAERLTQQYNELLHSLQSKMLNTSSLLEQLNDQFTWVSQLANLTQGDDQYLRVSTVTTHSSDSEVPSRVTEVVVKLFDSDPITVVLPEEVSKDNPKFMDTVAEKALQEYRRKSRMESGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV

Restriction Sites

SgfI-RsrII

Application Notes

The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.

Purification

Ion-exchange column purified

Research Areas

Neural Signal Transduction

Relevant Diseases

Alzheimer's Disease

Key Components

Transfection-ready dried plasmid DNA

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C

Research Use Only

For research use only

Target

Clusterin

Official Name

CLU

Full Name

Clusterin

Alternative Names

CLU; AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1; NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2; clusterin

Gene ID

1191(Human)

Uniprot ID

P10909(Human)