Creative Biolabs

Lipopolysaccharide (LPS)-induced Inflammation Assay

Lipopolysaccharide (LPS)-induced Inflammation Assay

Lipopolysaccharide (LPS) is considered an initiator of classical activation in monocytes and macrophages, its effects including an altered production of key mediators, such as inflammatory cytokines and chemokines. Inflammation within the central nervous system (CNS) is a common feature of neurological disorders and infections. With advanced technology and established in vitro assay platform, Creative Biolabs provides LPS-induced inflammation assay for multiple sclerosis (MS) research use.

LPS and MS

Lipopolysaccharide (LPS) is the major component of Gram-negative bacteria cell walls. LPS can cause an acute inflammatory response by triggering the release of a vast number of inflammatory cytokines in various cell types. LPS is widely recognized as a potent activator of monocytes and macrophages. Conventionally, bacterial LPS has been used to study inflammation, due to the abundance of inflammatory effects that it generates through TLR4 signaling. Extensive studies have shown that in monocytes/macrophages, LPS induces the production of cytokines such as IL1β, IL6, IL8, IL10, IL12, IL15, TNFα, and TGFβ.

MS is a chronic, disabling disease of the CNS that has an autoimmune component. Studies indicated that the changes in LPS and LPS-binding protein (LBP) are related to the development and evolution of MS. Moreover, antioxidant therapies may improve or block the development of neurodegenerative diseases, specifically MS, at least partly, through their effects on LBP.

Summarized downstream effects of LPS signaling.Fig.1 Summarized downstream effects of LPS signaling. (Page, et al., 2022)

Inflammatory Cytokine Immunoassay

Experimentally, inflammation can be induced in most tissues by the local injection of LPS, and typically large numbers of neutrophils and monocytes invade the tissue from the bloodstream within minutes, macrophages remaining at the site for several days. For in vitro assay, monocytes or macrophages could be preincubated for 2 hours in culture medium. Then, the monocytes and macrophages are activated with LPS for an additional 24 or 48 hours. After LPS activation, the conditioned media was centrifuged and kept at -20°C until ELISA analysis.

LPS-induced Inflammation Assay

Using a well-established cellular model of inflammation, Creative Biolabs helps you evaluate the potential of the drug in reducing the inflammatory effects induced by LPS in monocytes/macrophages. As your trustworthy drug discovery partner, get the data you need by choosing from our portfolio of standardized assays using pre-qualified primary stem cells or discussing your customized needs with our in-house experts.

Why Choose us?

  • Trusted for Quality: Our high standards for methods, materials, processes and customer communication are evident in the loyalty of our returning clientele.
  • Open Communication: As your eyes and ears in the lab, we place a priority on communication with our clients throughout the study process.
  • Extensive: We work directly with the scientists who develop the custom project used in our assays.

If you are interested in our in vitro assay, please feel free to contact us for more information.

Reference

  1. Page, M.J.; et al. The Role of Lipopolysaccharide-Induced Cell Signaling in Chronic Inflammation. Chronic Stress. 2022, 6: 24705470221076390.
For Research Use Only. Not For Clinical Use.
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