Creative Biolabs

Mesencephalic Tissue-Derived N27 Dopaminergic Neural Cell Line (Rat)

[CAT#: NCL200502ZP]

Used as a model of dopaminergic neurons for in vitro and in vivo studies.

Cell Types:

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Product Overview


SV40 was used to immortalize the N27 rat dopaminergic neuron cell line, which was isolated from E12 rat midbrain tissue. The neurological deficiency improved over time after the N27 cell line was introduced into the striatum of 6-hydroxydopamine-damaged rats (an animal model of PD). For both in vitro and in vivo research, this immortalized cell line can be employed as a model for dopaminergic neurons because it has been carefully characterized in dopamine biosynthesis and neurotoxicity investigations.

Cell Types


Relevant Diseases

Parkinson's Disease




Each vial contains ≥ 1X10e6 viable cells.


Frozen Cryovials/Live Culture

Tissue Source

E12 rat mesencephalic tissue


1RB3AN27 Cell Line

Cell Purity


Cell Viability


Mycoplasma Testing

Cells are negative for mycoplasma contamination.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.


Dry ice


Liquid nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.


Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

Mycoplasma was tested for sterility, post-freezing viability, and short-term repeat (STR) analysis for cell line identification. Cytochrome oxidase I (COI) was analyzed for cell line species identification.
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For Research Use Only. Not For Clinical Use.
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