Mesencephalic Tissue-Derived N27 Dopaminergic Neural Cell Line (Rat)
[CAT#: NCL200502ZP]
Used as a model of dopaminergic neurons for in vitro and in vivo studies.
- Species:
- Rat
- Cell Types:
- DopaNeuron
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
SV40 was used to immortalize the N27 rat dopaminergic neuron cell line, which was isolated from E12 rat midbrain tissue. The neurological deficiency improved over time after the N27 cell line was introduced into the striatum of 6-hydroxydopamine-damaged rats (an animal model of PD). For both in vitro and in vivo research, this immortalized cell line can be employed as a model for dopaminergic neurons because it has been carefully characterized in dopamine biosynthesis and neurotoxicity investigations.
Cell Types
DopaNeuron
Relevant Diseases
Parkinson's Disease
Species
Rat
Properties
Size
Each vial contains ≥ 1X10e6 viable cells.
Form
Frozen Cryovials/Live Culture
Tissue Source
E12 rat mesencephalic tissue
Strain
1RB3AN27 Cell Line
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
Cells are negative for mycoplasma contamination.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Mycoplasma was tested for sterility, post-freezing viability, and short-term repeat (STR) analysis for cell line identification. Cytochrome oxidase I (COI) was analyzed for cell line species identification.
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
