Creative Biolabs

Mesencephalic Tissue-Derived N27 Dopaminergic Neural Cell Line (Rat)

[CAT#: NCL200502ZP]

Used as a model of dopaminergic neurons for in vitro and in vivo studies.

Species:
Rat
Applications:
Cell Assay
Cell Types:
DopaNeuron

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Description
The N27 rat dopaminergic neuron cell line was harvested from E12 rat midbrain tissue and transfected with SV40 to immortalize it. When the N27 cell line was injected into the striatum of 6-hydroxydopamine-damaged rats (animal model of PD), it resulted in a time-dependent improvement in neurological deficit. This immortalized cell line has been carefully characterized in dopamine biosynthesis and neurotoxicity studies, and can be used as a model of dopaminergic neurons for in vitro and in vivo studies.
Cell Types
DopaNeuron
Applications
Cell Assay
Relevant Diseases
Parkinson's Disease; Neurological Disorders
Species
Rat
Size
Each vial contains ≥ 1X10e6 viable cells.
Form
Frozen Cryovials/Live Culture
Cell State
Live cells are in mid log-phase growth.
Tissue Source
E12 rat mesencephalic tissue
Strain
1RB3AN27 Cell Line
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
Cells are negative for mycoplasma contamination.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Mycoplasma was tested for sterility, post-freezing viability, and short-term repeat (STR) analysis for cell line identification. Cytochrome oxidase I (COI) was analyzed for cell line species identification.

For Research Use Only. Not For Clinical Use.
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